Journal article
Identification and characterization of a novel angiotensin binding site in cultured vascular smooth muscle cells that is specific for the hexapeptide (3–8) fragment of angiotensin II, angiotensin IV
Regulatory peptides, Vol.44(2), pp.225-232
1993
Handle:
https://hdl.handle.net/2376/113602
PMID: 8469776
Abstract
This study demonstrates the existence of a previously unrecognized class of angiotensin binding sites on vascular smooth muscle that exhibit high affinity and specificity for the hexapeptide (3–8) fragment of angiotensin II (AngIV). Binding of [
125I]AngIV is saturable, reversible and describes a pharmacologic profile that is distinct and separate from the classic AT
1 or AT
2 angiotensin receptors. Saturation binding studies utilizing cultured vascular smooth muscle cells obtained from bovine aorta (BVSM) revealed that [
125I]AngIV bound to a single high affinity site with an associated Hill coefficient of 0.99 ± 0.003, exhibiting a
K
D = 1.85 ± 0.45 nM and a corresponding
B
max = 960 ± 100 fmol mg
−1 protein. Competition binding curves in BVSM demonstrated the following rank order effectiveness: AngIV > AngII(3–7) ⪢ AngIII > Sar
1,Ile
8 AngII > AngII > AngII(1–7) > AngII(4–8), DuP 753, PD123177. The presence of the non-hydrolyzable GTP analog GTPγS, had no effect on [
125I]AngIV binding affinity in BVSM. The presence of this novel angiotensin binding site on smooth muscle in high concentration suggests the possibility that this system may play an important, yet unrecognized role in vascular control.
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Details
- Title
- Identification and characterization of a novel angiotensin binding site in cultured vascular smooth muscle cells that is specific for the hexapeptide (3–8) fragment of angiotensin II, angiotensin IV
- Creators
- Keith L Hall - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology College of Veterinary Medicine, Washington State University, Pullman, WA USAJodie M Hanesworth - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology College of Veterinary Medicine, Washington State University, Pullman, WA USAAmy E Ball - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology College of Veterinary Medicine, Washington State University, Pullman, WA USAGrant P Felgenhauer - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology College of Veterinary Medicine, Washington State University, Pullman, WA USAHoward L Hosick - Department of Genetics and Cell Biology, Washington State University, Pullman, WA USAJoseph W Harding - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology College of Veterinary Medicine, Washington State University, Pullman, WA USA
- Publication Details
- Regulatory peptides, Vol.44(2), pp.225-232
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548239401842
- Language
- English
- Resource Type
- Journal article