Journal article
Identification and characterization of monoclonal antibodies reactive with bovine, caprine and ovine T-lymphocyte determinants by flow microfluorimetry
Veterinary immunology and immunopathology, Vol.25(2), pp.195-208
1990
Handle:
https://hdl.handle.net/2376/114425
PMID: 1696040
Abstract
Comparative flow microfluorimetric (FMF) analysis was used to identify and characterize 27 monoclonal antibodies (MoAbs) reactive with bovine T-lymphocytes. Determinants present on all circulating T-lymphocytes were recognized by 11 MoAbs, 8 of which blocked E rosette formation. Determinants present on only the BoCD4
+ T-lymphocyte subset were detected by 9 MoAbs, while determinants restricted to the BoCD8
+ T-lymphocyte subset were recognized by 7 MoAbs. Competitive labeling experiments demonstrated that determinants recognized by subset-specific MoAbs were present on BoCD4 or BoCD8 molecules. Comparative studies revealed that some determinants, both pan-T specific and subset-specific, were conserved on homologous (orthologous) molecules expressed on leukocytes from other species of ruminants. Polymorphism was evident with several determinants.
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Details
- Title
- Identification and characterization of monoclonal antibodies reactive with bovine, caprine and ovine T-lymphocyte determinants by flow microfluorimetry
- Creators
- R.A Larsen - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman, WA 99164-7040 U.S.AM.L Monaghan - Department of Farm Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge Dublin 4 IrelandY.H Park - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman, WA 99164-7040 U.S.AM.J Hamilton - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman, WA 99164-7040 U.S.AJ.A Ellis - Departments of Veterinary Sciences and Molecular Biology, University of Wyoming, Laramie, WY U.S.AW.C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman, WA 99164-7040 U.S.A
- Publication Details
- Veterinary immunology and immunopathology, Vol.25(2), pp.195-208
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547321401842
- Language
- English
- Resource Type
- Journal article