Identification and characterization of testis- and epididymis-specific genes: cystatin SC and cystatin TE-1

Ying Li; Patrick J Friel; Monty O Robinson; Derek J McLean; Michael D Griswold

doi:10.1095/biolreprod.102.003368

Back

Identification and characterization of testis- and epididymis-specific genes: cystatin SC and cystatin TE-1

Journal article

Open access

Peer reviewed

Identification and characterization of testis- and epididymis-specific genes: cystatin SC and cystatin TE-1

Ying Li, Patrick J Friel, Monty O Robinson, Derek J McLean and Michael D Griswold

Biology of reproduction, Vol.67(6), pp.1872-1880

12/2002

DOI: https://doi.org/10.1095/biolreprod.102.003368

Handle:

https://hdl.handle.net/2376/107463

PMID: 12444065

Files and links (1)

url

https://doi.org/10.1095/biolreprod.102.003368View

Published (Version of record) Open

Abstract

Epithelial Cells - chemistry

Molecular Sequence Data

RNA, Messenger - analysis

Male

Gene Expression Profiling

Tissue Distribution

In Situ Hybridization

Base Sequence

Cloning, Molecular

Sertoli Cells - chemistry

Amino Acid Sequence

Rats

Organ Specificity

Reverse Transcriptase Polymerase Chain Reaction

Testis - growth & development

Rats, Sprague-Dawley

Liver - chemistry

Testis - chemistry

Blotting, Northern

Epididymis - chemistry

Sequence Alignment

Animals

Cystatins - genetics

Cystatins - chemistry

Brain Chemistry

Mice

Differential display-reverse transcriptase-polymerase chain reaction was used to examine Sertoli cell gene expression. As a result, two new members of the mouse cystatin multigene family were isolated and named cystatin SC (cystatin-related gene expressed in Sertoli cells) and cystatin TE-1 (cystatin-related gene highly expressed in testis and epididymis). The full-length cDNA sequence of cystatin SC contains an open reading frame that encodes a putative signal peptide of 20 amino acids and a mature protein of 110 amino acids, whereas that of cystatin TE-1 encodes a 128 amino acid protein with a predicted signal peptide of 21 amino acids. Both of the deduced amino acid sequences contain four highly conserved cysteine residues in precise alignment with other cystatin family members. The derived cystatin SC and TE-1 amino acid sequences lack some of the specific, highly conserved motifs believed to be necessary for cysteine proteinase inhibition activity. Northern blot analysis revealed that cystatin SC mRNA was detected only in the testis, whereas the cystatin TE-1 gene was highly expressed in testis and epididymis with very low expression in ovary and prostate. In situ hybridization showed that cystatin SC mRNA was localized mainly to Sertoli cells with an obvious stage-dependent expression, and that cystatin TE-1 mRNA was predominantly expressed in Sertoli cells without apparent stage-dependent expression. Cystatin TE-1 mRNA, as displayed by in situ hybridization, was expressed only in the epithelial cells of the proximal caput region of the epididymis. The unusual amino acid sequence and highly restricted expression suggests that cystatins SC and TE-1 play a very specialized role in the testis and epididymis.

Metrics

10 Record Views

Details

Title: Identification and characterization of testis- and epididymis-specific genes: cystatin SC and cystatin TE-1
Creators: Ying Li - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman 99164-4660, USA
Patrick J Friel
Monty O Robinson
Derek J McLean
Michael D Griswold
Publication Details: Biology of reproduction, Vol.67(6), pp.1872-1880
Academic Unit: Center for Reproductive Biology; Molecular Biosciences, School of
Publisher: United States
Grant note: HD 10808 / NICHD NIH HHS
Identifiers: 99900547016101842
Language: English
Resource Type: Journal article