Journal article
Identification and function of a polyketide synthase gene responsible for 1,8-dihydroxynaphthalene-melanin pigment biosynthesis in Ascochyta rabiei
Current genetics, Vol.56(4), pp.349-360
08/2010
Handle:
https://hdl.handle.net/2376/105476
PMID: 20473673
Abstract
Ascochyta rabiei produces and accumulates one of the well-known fungal polyketides, 1,8-dihydroxynaphthalene-melanin pigment (DHN-melanin), in asexual and sexual fruiting bodies. Degenerate PCR primers were used to isolate an ArPKS1 of A. rabiei encoding a polypeptide with high similarity to polyketide synthase (PKS) involved in biosynthesis of DHN-melanin in other ascomycetous fungi. Site-directed mutagenesis of ArPKS1 in A. rabiei generated melanin-deficient pycnidial mutants but caused no significant reduction of pathogenicity to chickpea. Pycnidiospores in ArPKS1-mutant pycnidia showed higher sensitivity to UV light exposure compared to pycnidiospores in melanized pycnidia of the wild-type progenitor isolate. Integration of an orthologous PKS1 gene from Bipolaris oryzae into the genome of the mutants complemented the dysfunctional ArPKS1 gene. This study demonstrated that A. rabiei uses a DHN-melanin pathway for pigmentation of pycnidia and this molecule may protect pycnidiospores from UV irradiation.
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Details
- Title
- Identification and function of a polyketide synthase gene responsible for 1,8-dihydroxynaphthalene-melanin pigment biosynthesis in Ascochyta rabiei
- Creators
- Hajime O Akamatsu - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. akamatho@affrc.go.jpMartin I ChilversJane E StewartTobin L Peever
- Publication Details
- Current genetics, Vol.56(4), pp.349-360
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546624901842
- Language
- English
- Resource Type
- Journal article