Journal article
Identification and localization of eight distinct hormone-producing cell types in the pituitary of male Atlantic halibut ( Hippoglossus hippoglossus L.)
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol.134(2), pp.315-327
2003
Handle:
https://hdl.handle.net/2376/104667
PMID: 12547261
Abstract
The eight distinct hormone-producing cell types in the adenohypophysis of male Atlantic halibut (
Hippoglossus hippoglossus L.) were identified and localized using immunohistochemistry and in situ hybridization. Lactotropes either occupied most of the rostral pars distalis (RPD) or they were arranged in follicular structures located along the periphery of the RPD. Corticotropes were confined to a thin layer of RPD cells bordering the pars nervosa (PN). The somatotropes were arranged in multicellular layers bordering the highly convoluted PN penetrating the proximal pars distalis (PPD), while thyrotropes, scattered in small islets in between the somatotropes, were located in the centro-dorsal part of the PPD. Gonadotropes were found throughout the PPD. Immunoreactivity to glycoprotein-α and luteinizing hormone β-subunit was also observed along the periphery of the pars intermedia (PI), indicating that a thin extension of the PPD surrounded the PI. In situ hybridization showed that follicle-stimulating hormone and luteinizing hormone were produced in distinct cells of the PPD. PI contained somatolactotropes bordering the highly convoluted PN, and melanotropes that showed positive immunostaining against both anti-α-melanocyte-stimulating hormone and anti-β-endorphin. The general cellular organization was similar to that of other teleost fish. These results lay the basis for future investigations on Atlantic halibut pituitary physiology.
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Details
- Title
- Identification and localization of eight distinct hormone-producing cell types in the pituitary of male Atlantic halibut ( Hippoglossus hippoglossus L.)
- Creators
- Finn-Arne Weltzien - Institute of Marine Research, Austevoll Aquaculture Research Station, 5392 Storebo, NorwayBirgitta Norberg - Institute of Marine Research, Austevoll Aquaculture Research Station, 5392 Storebo, NorwayJon Vidar Helvik - Department of Molecular Biology, University of Bergen, P.O. Box 7800, 5020 Bergen, NorwayØivind Andersen - Institute of Aquaculture Research, P.O. Box 5010, 1432 Aas, NorwayPenny Swanson - Northwest Fisheries Science Center, NMFS, 2725 Montlake Boulevard East, Seattle, WA 98112, USAEva Andersson - Department of Fisheries and Marine Biology, University of Bergen, P.O. Box 7800, 5020 Bergen, Norway
- Publication Details
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol.134(2), pp.315-327
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Inc
- Identifiers
- 99900546554401842
- Language
- English
- Resource Type
- Journal article