Journal article
Identification of Africanized Honey Bee (Hymenoptera: Apidae) Mitochondrial DNA: Validation of a Rapid Polymerase Chain Reaction-Based Assay
Annals of the Entomological Society of America, Vol.96(5), pp.679-684
09/01/2003
Handle:
https://hdl.handle.net/2376/113907
Abstract
Polymerase chain reaction (PCR)-amplified mitochondrial DNA (mtDNA) assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identification of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (Bgl II) of a single PCR-amplified segment of the cytochrome b gene. The Bgl II polymorphism discriminates the mitochondrial haplotype (mitotype) of Apis mellifera scutellata L. (ancestor of Africanized bees) from that of A. m. mellifera , A. m. caucasia , A. m. ligustica , A. m. carnica , A. m. lamarcki , A. m. cypria , A. m. syriaca , and some A. m. iberiensis , but not from that of A. m. intermissa and some A. m. iberiensis . Nonetheless, given the very low frequency (<1%) of African non-A. m. scutellata mitotype present before arrival of Africanized bees in the United States, cytochrome b/Bgl II assay can be used to identify maternally Africanized bees with a high degree of reliability.
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Details
- Title
- Identification of Africanized Honey Bee (Hymenoptera: Apidae) Mitochondrial DNA: Validation of a Rapid Polymerase Chain Reaction-Based Assay
- Creators
- M A PintoJ S JohnstonW L RubinkR N CoulsonJ C PattonW S Sheppard
- Publication Details
- Annals of the Entomological Society of America, Vol.96(5), pp.679-684
- Identifiers
- 99900548035901842
- Language
- English
- Resource Type
- Journal article