Journal article
Identification of Anaplasma marginale Outer Membrane Protein Antigens Conserved between A. marginale Sensu Stricto Strains and the Live A. marginale subsp. centrale Vaccine
Infection and immunity, Vol.79(3), pp.1311-1318
03/2011
Handle:
https://hdl.handle.net/2376/100961
PMCID: PMC3067503
PMID: 21189322
Abstract
Live vaccination with
Anaplasma marginale
subsp.
centrale
(synonym for
Anaplasma centrale
) induces protection against severe disease upon challenge with
A. marginale
sensu stricto strains. Despite over a century of field use, the targets of protective immunity remained unknown. Using a broad proteomic approach, we identified the proteins in a challenge sensu stricto strain that were bound by the relevant antibody isotype induced by live vaccination with
Anaplasma marginale
subsp.
centrale.
A core of 15 proteins was identified in vaccinated animals across multiple major histocompatibility complex (MHC) haplotypes. This core separated into two structural/functional classes: “housekeeping” proteins involved in replication and metabolism and outer membrane proteins (OMPs). Orthologous proteins of both classes were identified within the vaccine strain and among sensu stricto strains. In contrast to the broad conservation among strains in the sequences of the housekeeping proteins, there was significantly greater divergence in the OMPs and greater divergence in both OMP sequences and the encoding locus structure between the vaccine strain and the sensu stricto strains than among the sensu stricto strains. The OMPs bound by live vaccine-induced antibody overlapped with OMPs that were immunogenic in animals vaccinated with inactivated vaccines and subsequently protected against bacteremia and disease. The identification of this core set of OMPs is consistent with the hypothesis that “subdominant” immunogens are required for vaccine-induced protection against
A. marginale
and provides clear direction for development of a safer, more effective vaccine.
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Details
- Title
- Identification of Anaplasma marginale Outer Membrane Protein Antigens Conserved between A. marginale Sensu Stricto Strains and the Live A. marginale subsp. centrale Vaccine
- Creators
- Joseph T Agnes - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Kelly A Brayton - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Megan LaFollett - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Junzo Norimine - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Wendy C Brown - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Guy H Palmer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.79(3), pp.1311-1318
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546552901842
- Language
- English
- Resource Type
- Journal article