Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector

Solomon S Ramabu; Massaro W Ueti; Kelly A Brayton; Timothy V Baszler; Guy H Palmer

doi:10.1128/IAI.00300-10

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Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector

Journal article

Open access

Peer reviewed

Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector

Solomon S Ramabu, Massaro W Ueti, Kelly A Brayton, Timothy V Baszler and Guy H Palmer

Infection and immunity, Vol.78(7), pp.3047-3052

07/2010

DOI: https://doi.org/10.1128/IAI.00300-10

Handle:

https://hdl.handle.net/2376/112064

PMCID: PMC2897376

PMID: 20439479

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https://doi.org/10.1128/IAI.00300-10View

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Abstract

Up-Regulation

Electrophoresis, Gel, Two-Dimensional

Arachnid Vectors - microbiology

Bacterial Proteins - physiology

Blotting, Western

Gene Expression Regulation, Bacterial - physiology

Dermacentor - microbiology

Anaplasmosis - microbiology

Anaplasma marginale - physiology

Animals

Bacterial Proteins - biosynthesis

Bacterial Proteins - isolation & purification

Life Cycle Stages - physiology

Proteomics

The transition between infection of the mammalian host and colonization of an arthropod vector is required for the ongoing transmission of a broad array of pathogens, from viruses to protozoa. Understanding how this transition is mediated provides opportunities to disrupt transmission through either chemotherapy or immunization. We used an unbiased proteomic screen to identify Anaplasma marginale proteins specifically upregulated in the tick compared to the mammalian host. Comparative mass spectrometric analysis of proteins separated by two-dimensional gel electrophoresis of uninfected and infected ISE6 cells and infected mammalian cells identified 15 proteins exclusively expressed or upregulated in tick cells. All 15 had originally been annotated as hypothetical proteins. We confirmed quantitative upregulation and expression in situ within the midgut epithelial and salivary gland acinar cells of vector ticks during successful transmission. The results support the hypothesis that A. marginale gene expression is regulated by the specific host environment and, in a broader context, that the core genome evolved in the arthropod vector with differential regulation, allowing adaptation to mammalian hosts. Furthermore, the confirmation of the in situ expression of candidates identified in ISE6 cell lines indicates that this approach may be widely applicable to bacteria in the genera Anaplasma and Ehrlichia, removing a major technical impediment to the identification of new targets for vaccine and chemotherapeutic blocking of transmission.

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Title: Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector
Creators: Solomon S Ramabu - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology and School for Global Animal Health, Washington State University, Pullman, Washington 99164-7040, USA
Massaro W Ueti
Kelly A Brayton
Timothy V Baszler
Guy H Palmer
Publication Details: Infection and immunity, Vol.78(7), pp.3047-3052
Academic Unit: Paul G. Allen School for Global Animal Health; Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: R37 AI044005 / NIAID NIH HHS GR075800M / Wellcome Trust R01 AI044005 / NIAID NIH HHS AI44005 / NIAID NIH HHS
Identifiers: 99900547327301842
Language: English
Resource Type: Journal article