Journal article
Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens
Applied and environmental microbiology, Vol.74(22), pp.6867-6875
11/2008
Handle:
https://hdl.handle.net/2376/106382
PMCID: PMC2583476
PMID: 18805999
Abstract
Campylobacter jejuni
is one of the leading bacterial causes of food-borne gastroenteritis. Infection with
C. jejuni
is frequently acquired through the consumption of undercooked poultry or foods cross-contaminated with raw poultry. Given the importance of poultry as a reservoir for
Campylobacter
organisms, investigators have performed studies to understand the protective role of maternal antibodies in the ecology of
Campylobacter
colonization of poultry. In a previous study, chicks with maternal antibodies generated against the S3B strain of
C. jejuni
provided protection against
Campylobacter
colonization (O. Sahin, N. Luo, S. Huang, and Q. Zhang, Appl. Environ. Microbiol. 69:5372-5379, 2003). We obtained serum samples, collectively referred to as the
C. jejuni
S3B-SPF sera, from the previous study. These sera were determined to contain maternal antibodies that reacted against
C. jejuni
whole-cell lysates as judged by enzyme-linked immunosorbent assay. The antigens recognized by the
C. jejuni
S3B-SPF antibodies were identified by immunoblot analysis, coupled with mass spectrometry, of
C. jejuni
outer membrane protein extracts. This approach led to the identification of
C. jejuni
proteins recognized by the maternal antibodies, including the flagellin proteins and CadF adhesin. In vitro assays revealed that the
C. jejuni
S3B-SPF sera retarded the motility of the
C. jejuni
S3B homologous strain but did not retard the motility of a heterologous strain of
C. jejuni
(81-176). This finding provides a possible mechanism explaining why maternal antibodies confer enhanced protection against challenge with a homologous strain compared to a heterologous strain. Collectively, this study provides a list of
C. jejuni
proteins against which protective antibodies are generated in hens and passed to chicks.
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Details
- Title
- Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens
- Creators
- Kari D Shoaf-Sweeney - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234Charles L Larson - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234Xiaoting Tang - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234Michael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234
- Publication Details
- Applied and environmental microbiology, Vol.74(22), pp.6867-6875
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546933001842
- Language
- English
- Resource Type
- Journal article