Identification of Lygus hesperus by DNA barcoding reveals insignificant levels of genetic structure among distant and habitat diverse populations

Changqing Zhou; Irfan Kandemir; Douglas B Walsh; Frank G Zalom; Laura Corley Lavine

doi:10.1371/journal.pone.0034528

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Identification of Lygus hesperus by DNA barcoding reveals insignificant levels of genetic structure among distant and habitat diverse populations

Journal article

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Identification of Lygus hesperus by DNA barcoding reveals insignificant levels of genetic structure among distant and habitat diverse populations

Changqing Zhou, Irfan Kandemir, Douglas B Walsh, Frank G Zalom and Laura Corley Lavine

PloS one, Vol.7(3), pp.e34528-e34528

2012

DOI: https://doi.org/10.1371/journal.pone.0034528

Handle:

https://hdl.handle.net/2376/117008

PMCID: PMC3316671

PMID: 22479640

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Abstract

DNA - genetics

DNA - isolation & purification

Animals

Heteroptera - genetics

United States

Ecosystem

Fragaria - parasitology

Medicago sativa - parasitology

Phylogeny

DNA Barcoding, Taxonomic

Genetic Structures

The western tarnished plant bug Lygus hesperus is an economically important pest that belongs to a complex of morphologically similar species that makes identification problematic. The present study provides evidence for the use of DNA barcodes from populations of L. hesperus from the western United States of America for accurate identification. This study reports DNA barcodes for 134 individuals of the western tarnished plant bug from alfalfa and strawberry agricultural fields in the western United States of America. Sequence divergence estimates of <3% reveal that morphologically variable individuals presumed to be L. hesperus were accurately identified. Paired estimates of F(st) and subsequent estimates of gene flow show that geographically distinct populations of L. hesperus are genetically similar. Therefore, our results support and reinforce the relatively recent (<100 years) migration of the western tarnished plant bug into agricultural habitats across the western United States. This study reveals that despite wide host plant usage and phenotypically plastic morphological traits, the commonly recognized western tarnished plant bug belongs to a single species, Lygus hesperus. In addition, no significant genetic structure was found for the geographically diverse populations of western tarnished plant bug used in this study.

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Title: Identification of Lygus hesperus by DNA barcoding reveals insignificant levels of genetic structure among distant and habitat diverse populations
Creators: Changqing Zhou - Department of Entomology, Washington State University, Pullman, Washington, United States of America
Irfan Kandemir
Douglas B Walsh
Frank G Zalom
Laura Corley Lavine
Publication Details: PloS one, Vol.7(3), pp.e34528-e34528
Academic Unit: WSU Prosser IAREC; Entomology, Department of
Publisher: United States
Identifiers: 99900547954401842
Language: English
Resource Type: Journal article