Journal article
Identification of Novel Antigenic Proteins in a Complex Anaplasma marginale Outer Membrane Immunogen by Mass Spectrometry and Genomic Mapping
Infection and immunity, Vol.73(12), pp.8109-8118
12/2005
Handle:
https://hdl.handle.net/2376/114253
PMCID: PMC1307060
PMID: 16299305
Abstract
Immunization with purified
Anaplasma marginale
outer membranes induces complete protection against infection that is associated with CD4
+
T-lymphocyte-mediated gamma interferon secretion and immunoglobulin G2 (IgG2) antibody titers. However, knowledge of the composition of the outer membrane immunogen is limited. Recent sequencing and annotation of the
A. marginale
genome predicts at least 62 outer membrane proteins (OMP), enabling a proteomic and genomic approach for identification of novel OMP by use of IgG serum antibody from outer membrane vaccinates. Outer membrane proteins were separated by two-dimensional electrophoresis, and proteins recognized by total IgG and IgG2 in immune sera of outer membrane-vaccinated cattle were detected by immunoblotting. Immunoreactive protein spots were excised and subjected to liquid chromatography-tandem mass spectrometry. A database search of the
A. marginale
genome identified 24 antigenic proteins that were predicted to be outer membrane, inner membrane, or membrane-associated proteins. These included the previously characterized surface-exposed outer membrane proteins MSP2, operon associated gene 2 (OpAG2), MSP3, and MSP5 as well as recently identified appendage-associated proteins. Among the 21 newly described antigenic proteins, 14 are annotated in the
A. marginale
genome and include type IV secretion system proteins, elongation factor Tu, and members of the MSP2 superfamily. The identification of these novel antigenic proteins markedly expands current understanding of the composition of the protective immunogen and provides new candidates for vaccine development.
Metrics
8 Record Views
Details
- Title
- Identification of Novel Antigenic Proteins in a Complex Anaplasma marginale Outer Membrane Immunogen by Mass Spectrometry and Genomic Mapping
- Creators
- Job E Lopez - Department of Veterinary Microbiology and PathologyWilliam F Siems - Department of Veterinary Microbiology and PathologyGuy H Palmer - Department of Veterinary Microbiology and PathologyKelly A Brayton - Department of Veterinary Microbiology and PathologyTravis C McGuire - Department of Veterinary Microbiology and PathologyJunzo Norimine - Department of Veterinary Microbiology and PathologyWendy C Brown - Department of Veterinary Microbiology and Pathology
- Publication Details
- Infection and immunity, Vol.73(12), pp.8109-8118
- Academic Unit
- Chemistry, Department of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547955601842
- Language
- English
- Resource Type
- Journal article