Identification of a Campylobacter jejuni-secreted protein required for maximal invasion of host cells

Jeffrey E Christensen; Sophia A Pacheco; Michael E Konkel

doi:10.1111/j.1365-2958.2009.06797.x

Back

Identification of a Campylobacter jejuni-secreted protein required for maximal invasion of host cells

Journal article

Open access

Peer reviewed

Identification of a Campylobacter jejuni-secreted protein required for maximal invasion of host cells

Jeffrey E Christensen, Sophia A Pacheco and Michael E Konkel

Molecular microbiology, Vol.73(4), pp.650-662

08/2009

DOI: https://doi.org/10.1111/j.1365-2958.2009.06797.x

Handle:

https://hdl.handle.net/2376/108919

PMCID: PMC2764114

PMID: 19627497

Files and links (1)

url

https://doi.org/10.1111/j.1365-2958.2009.06797.xView

Published (Version of record) Open

Abstract

Cell Line

Genes, Bacterial

Virulence Factors - genetics

Campylobacter jejuni - pathogenicity

Humans

Bacterial Proteins - genetics

Campylobacter Infections - microbiology

Mutagenesis

Yersinia enterocolitica - metabolism

Campylobacter jejuni - metabolism

Bacterial Proteins - metabolism

Virulence Factors - metabolism

Campylobacter jejuni - genetics

The food-borne pathogen Campylobacter jejuni is dependent on a functional flagellum for motility and the export of virulence proteins that promote maximal host cell invasion. Both the flagellar and non-flagellar proteins exported via the flagellar type III secretion system contain a sequence within the amino-terminus that directs their export from the bacterial cell. Accordingly, we developed a genetic screen to identify C. jejuni genes that encode a type III secretion amino-terminal sequence that utilizes the flagellar type III secretion system of Yersinia enterocolitica and a phospholipase reporter (yplA). We screened a library of 321 C. jejuni genes and identified proteins with putative type III secretion amino-terminal sequences. One gene identified by the screen was Cj1242. We generated a mutation in Cj1242, and performed growth rate, motility, secretion and INT 407 cell adherence and internalization assays. The C. jejuni Cj1242 mutant was not altered in growth rate or motility when compared with the wild-type strain, but displayed an altered secretion profile and a reduction in host cell internalization. Based on the phenotype of the C. jejuni Cj1242 mutant, we designated the protein Campylobacter invasion antigen C (CiaC). Collectively, our findings indicate that CiaC is a potentially important virulence factor.

Metrics

8 Record Views

Details

Title: Identification of a Campylobacter jejuni-secreted protein required for maximal invasion of host cells
Creators: Jeffrey E Christensen - Department of Microbiology, School of Molecular Biosciences, Washington State University, Pullman, Washington, USA
Sophia A Pacheco
Michael E Konkel
Publication Details: Molecular microbiology, Vol.73(4), pp.650-662
Academic Unit: Molecular Biosciences, School of
Publisher: England
Grant note: N01 AI030055 / NIAID NIH HHS N01AI30055 / NIAID NIH HHS
Identifiers: 99900547120001842
Language: English
Resource Type: Journal article