Journal article
Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization
Genetics (Austin), Vol.184(3), pp.813-825
03/2010
Handle:
https://hdl.handle.net/2376/112173
PMCID: PMC2845348
PMID: 20176981
Abstract
Potentially useful naturally occurring genetic variation is often difficult to identify as the effects of individual genes are subtle and difficult to observe. In this study, a novel genetic technique called Mutant-Assisted Gene Identification and Characterization is used to identify naturally occurring loci modulating the hypersensitive defense response (HR) in maize. Mutant-Assisted Gene Identification and Characterization facilitates the identification of naturally occurring alleles underlying phenotypic variation from diverse germplasm, using a mutant phenotype as a “reporter.” In this study the reporter phenotype was caused by a partially dominant autoactive disease resistance gene,
Rp1-D21
, which caused HR lesions to form spontaneously all over the plant. Here it is demonstrated that the
Rp1-D21
phenotype is profoundly affected by genetic background. By crossing the
Rp1-D21
gene into the IBM mapping population, it was possible to map and identify
Hrml
1 on chromosome 10, a locus responsible for modulating the HR phenotype conferred by
Rp1-D21
. Other loci with smaller effects were identified on chromosomes 1 and 9. These results demonstrate that Mutant-Assisted Gene Identification and Characterization is a viable approach for identifying naturally occurring useful genetic variation.
Metrics
11 Record Views
Details
- Title
- Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization
- Creators
- Satya Chintamanani - Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054Scot H Hulbert - Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054Gurmukh S Johal - Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054Peter J Balint-Kurti - Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907–2054
- Publication Details
- Genetics (Austin), Vol.184(3), pp.813-825
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Genetics Society of America
- Identifiers
- 99900547462901842
- Language
- English
- Resource Type
- Journal article