Identification of a nonsense mutation in feline ABCB1

K L Mealey; N S Burke

doi:10.1111/jvp.12212

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Identification of a nonsense mutation in feline ABCB1

Journal article

Peer reviewed

Identification of a nonsense mutation in feline ABCB1

K L Mealey and N S Burke

Journal of veterinary pharmacology and therapeutics, Vol.38(5), pp.429-433

10/2015

DOI: https://doi.org/10.1111/jvp.12212

Handle:

https://hdl.handle.net/2376/107074

PMID: 25660379

Abstract

Imidazoles - adverse effects

Cats - genetics

Neonicotinoids

Exons - genetics

Nitro Compounds - adverse effects

Cat Diseases - chemically induced

Praziquantel - adverse effects

Homozygote

Macrolides - adverse effects

Animals

Cats - metabolism

Central Nervous System Diseases - chemically induced

Cloning, Molecular

Sequence Analysis, DNA - veterinary

ATP Binding Cassette Transporter, Sub-Family B - metabolism

Polymorphism, Single Nucleotide

Ivermectin - adverse effects

ATP Binding Cassette Transporter, Sub-Family B - genetics

Codon, Nonsense - genetics

ATP Binding Cassette Transporter, Sub-Family B - drug effects

Depsipeptides - adverse effects

The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats that experienced central nervous system toxicosis after receiving ivermectin (n = 2), a combination product containing moxidectin and imidacloprid (n = 3), a combination product containing praziquantel and emodepside (n = 1) or selamectin (n = 2), and 1 cat that received the product containing praziquantel and emodepside but did not experience toxicity (n = 1). Fifteen exons contained polymorphisms and twelve exons showed no variation from the reference sequence. The most significant finding was a nonsense mutation (ABCB11930_1931del TC) in one of the ivermectin-treated cats. This cat was homozygous for the deletion mutation. All of the other phenotyped cats were homozygous for the wild-type allele. However, 14 missense mutations were identified in one or more phenotyped cats. ABCB11930_1931del TC was also identified in four nonphenotyped cats (one homozygous and three heterozygous for the mutant allele). Cats affected by ABCB11930_1931del TC would be expected to have a similar phenotype as dogs with the previously characterized ABCB1-1Δ mutation.

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Title: Identification of a nonsense mutation in feline ABCB1
Creators: K L Mealey - Program in Individualized Medicine, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA
N S Burke - Program in Individualized Medicine, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA
Publication Details: Journal of veterinary pharmacology and therapeutics, Vol.38(5), pp.429-433
Academic Unit: Veterinary Clinical Sciences, Department of
Publisher: England
Identifiers: 99900547500801842
Language: English
Resource Type: Journal article

Identification of a nonsense mutation in feline ABCB1

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