Journal article
Identification of an AII(3–8) [AIV] binding site in guinea pig hippocampus
Brain research, Vol.583(1), pp.340-343
1992
Handle:
https://hdl.handle.net/2376/112262
PMID: 1504842
Abstract
A unique angiotensin binding site specific for the hexapeptide, AII(3–8), has been identified in guinea pig hippocampus. This binding site, which is present in the pyramidal cell layer of CA1, CA2, CA3 of the hippocampus and dentate gyrus, binds AII(3–8) with high affinity (
K
D
= 1.29 ± 0.18 nM) in a saturable manner (
B
max = 449 ± 62 fmol/mg protein). The N-terminal structure of the binding ligand is paramount in determining the binding affinity. The C-terminal requirements seem less stringent as evidenced by the binding affinity of AII(3–7) (
K
D
= 20.9 ± 2.1 nM). Neither AII, AIII, Sar
1, Ile
8-AII, Dup 753 nor CGP42112A appear to bind, indicating that this binding site is neither the AT1 nor AT2 sites described for AII/AIII. Autoradiographic analysis of hippocampus binding confirms the inability of Sar
1, Ile
8-AII to compete for [
125I]AII(3–8) binding. Conversely AII(3–8) was unable to displace [
125I]Sar
1,Ile
8-AII binding.
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Details
- Title
- Identification of an AII(3–8) [AIV] binding site in guinea pig hippocampus
- Creators
- Joseph W Harding - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Victoria I Cook - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Allison V Miller-Wing - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Jodie M Hanesworth - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Michael F Sardinia - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Keith L Hall - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Jeff W Stobb - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Geoff N Swanson - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)John K.M Coleman - Department of Psychology, Washington State University, Pullman, WA 99164-6520 (USA)John W Wright - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)Erin C Harding - Department of Veterinary and Comparative Anatomy, Pharmacology, Pullman, WA 99164-6520 (USA)
- Publication Details
- Brain research, Vol.583(1), pp.340-343
- Academic Unit
- Psychology, Department of; Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547669901842
- Language
- English
- Resource Type
- Journal article