Journal article
Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor
PloS one, Vol.13(10), pp.e0204831-e0204831
2018
Handle:
https://hdl.handle.net/2376/116045
PMCID: PMC6173382
PMID: 30289885
Abstract
Proteins, metabolites, and 16S rRNA measurements were used to examine the community structure and functional relationships within a cellulose degrading anaerobic bioreactor. The bioreactor was seeded with bovine rumen fluid and operated with a 4 day hydraulic retention time on cellulose (avicel) as sole carbon and energy source. The reactor performance and microbial community structure was monitored during the establishment of the cellulose-degrading community. After stable operation was established in the bioreactor, the mixing intensity was increased in order to investigate the effect of a physical disruption of the microbial community structure. Finally, the original conditions were re-established to understand the stability of the microbial community after a perturbation. All factors measured were found to be inter-correlated during these three distinct phases of operation (establishment, perturbation and re-establishment). In particular, the return of community structure and function to pre-perturbed conditions suggests that propionate fermentation and acetate utilization were the explanatory factors for community establishment and re-establishment.
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Details
- Title
- Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor
- Creators
- Stephen J Callister - Department of Biological Systems Engineering, Washington State University, Pullman, Washington, United States of AmericaLee Ann McCue - Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of AmericaAmy A Boaro - Bioproducts, Sciences and Engineering Laboratory, Washington State University Tri-Cities, Richland, Washington, United States of AmericaBrian LaMarche - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of AmericaRichard A White, 3rd - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of AmericaJoseph M Brown - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of AmericaBirgitte K Ahring - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, United States of America
- Publication Details
- PloS one, Vol.13(10), pp.e0204831-e0204831
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- United States
- Identifiers
- 99900547904701842
- Language
- English
- Resource Type
- Journal article