Journal article
Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale
Infection and immunity, Vol.75(6), pp.2959-2964
06/2007
Handle:
https://hdl.handle.net/2376/112446
PMCID: PMC1932854
PMID: 17420231
Abstract
Understanding the determinants of efficient tick-borne microbial transmission is needed to better predict the emergence of highly transmissible pathogen strains and disease outbreaks. Although the basic developmental cycle of Anaplasma and Ehrlichia spp. within the tick has been delineated, there are marked differences in the ability of specific strains to be efficiently tick transmitted. Using the highly transmissible St. Maries strain of Anaplasma marginale in Dermacentor andersoni as a positive control and two unrelated nontransmissible strains, we identified distinct barriers to efficient transmission within the tick. The Mississippi strain was unable to establish infection at the level of the midgut epithelium despite successful ingestion of infected blood following acquisition feeding on a bacteremic animal host. This inability to colonize the midgut epithelium prevented subsequent development within the salivary glands and transmission. In contrast, A. marginale subsp. centrale colonized the midgut and then the salivary glands, replicating to a titer indistinguishable from that of the highly transmissible St. Maries strain and at least 100 times greater than that previously associated with successful transmission. Nonetheless, A. marginale subsp. centrale was not transmitted, even when a large number of infected ticks was used for transmission feeding. These results establish that there are at least two specific barriers to efficient tick-borne transmission, the midgut and salivary glands, and highlight the complexity of the pathogen-tick interaction.
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Details
- Title
- Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale
- Creators
- Massaro W Ueti - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. massaro@vetmed.wsu.eduJames O Reagan, JrDonald P Knowles, JrGlen A ScolesVarda ShkapGuy H Palmer
- Publication Details
- Infection and immunity, Vol.75(6), pp.2959-2964
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- T32 AI007025 / NIAID NIH HHS GR075800M / Wellcome Trust R01 AI044005 / NIAID NIH HHS R01 AI 44005 / NIAID NIH HHS T32 AI 007025 / NIAID NIH HHS
- Identifiers
- 99900547625501842
- Language
- English
- Resource Type
- Journal article