Journal article
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues
Canadian journal of veterinary research, Vol.60(3), pp.193-198
07/1996
Handle:
https://hdl.handle.net/2376/105060
PMCID: PMC1263832
PMID: 8809382
Abstract
Commercially-available monoclonal antibodies to B lymphocytes were evaluated for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed, paraffin-embedded tissues using an avidin biotin complex immunoperoxidase immunohistochemical technique. Three monoclonal antibodies: F46A and F72A raised to "carnivore" B lymphocytes and RA3.6B2 raised to murine B lymphocytes, stained B lymphocyte-dependent areas of frozen feline lymphoid tissue. In addition, antibody RA3.6B2 stained B lymphocyte dependent areas in formalin-fixed, paraffin-embedded feline tissues. There was no staining of T lymphocyte-dependent areas in either frozen or formalin-fixed tissues. Dual parameter flow cytometry, using an anti-pan-T lymphocyte antibody, revealed that greater than 99% of the cells stained by RA3.6B2 are a population distinct from T lymphocytes. F46A was shown to stain a sub-population of those cells stained with RA3.6B2. These antibodies may be useful in the identification of feline B lymphocytes using immunohistochemistry and flow cytometry and thereby provide additional tools to study B lymphocyte ontogeny and the significance of lymphocyte phenotype in lymphoid neoplasia in cats.
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Details
- Title
- Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues
- Creators
- C E Monteith - Department of Pathology, Western College of Veterinary Medicine, Saskatoon, SaskatchewanB J ChelackW C DavisD M Haines
- Publication Details
- Canadian journal of veterinary research, Vol.60(3), pp.193-198
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Canada
- Identifiers
- 99900546550601842
- Language
- English
- Resource Type
- Journal article