Journal article
Identification of target amino acids that affect interactions of fungal polygalacturonases and their plant inhibitors
Physiological and molecular plant pathology, Vol.56(3), pp.117-130
03/2000
Handle:
https://hdl.handle.net/2376/116202
Abstract
Plant polygalacturonase inhibitor proteins (PGIPs) bind fungal polygalacturonases (PGs), but inhibition specificities and kinetics vary within and among species. Purified bean PGIP inhibited all fungal PGs we tested, includingFusarium moniliforme PG. Pear PGIP, however, was only effective against Botrytis cinerea PG. Moreover, tomato PGIP inhibited B. cinerea PG more than Aspergillus niger PG. Models of codon evolution for 22 dicot PGIPs and 19 fungal PGs indicated that advantageous substitutions dominate the molecular evolution of these genes and identified 9 amino acid residues, each, that are likely to evolve adaptively in response to natural selection. Many of these residues are within the β-strand/β-turn region of the PGIP LRR, including two sites known to alter inhibition specificities of bean PGIPs, but others lie outside this region. Our results complement existing molecular and biochemical studies of resistance specificity, and suggest new target amino acids for manipulating PG-inhibition.
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Details
- Title
- Identification of target amino acids that affect interactions of fungal polygalacturonases and their plant inhibitors
- Creators
- HENRIK U STOTZ - Max-Planck-Institute of Chemical Ecology, Carl-Zeiss Promenade 10, D-07745, Jena, GermanyJOHN G BISHOP - School of Biological Sciences, Washington State University, Vancouver, WA, 98686, U.S.ACARL W BERGMANN - Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602-4712, U.S.AMARCUS KOCH - Institute of Systematic Botany and Geobotany, Gregor-Mendel-Str. 33, A-1180, Vienna, AustriaPETER ALBERSHEIM - Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602-4712, U.S.AALAN G DARVILL - Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602-4712, U.S.AJOHN M LABAVITCH - Department of Pomology, University of California, Davis, CA, 95616, U.S.A
- Publication Details
- Physiological and molecular plant pathology, Vol.56(3), pp.117-130
- Academic Unit
- Biological Sciences, School of
- Publisher
- Elsevier India Pvt Ltd
- Identifiers
- 99900547334601842
- Language
- English
- Resource Type
- Journal article