Journal article
Identification of the Enteropathogens Campylobacter jejuni and Campylobacter coli Based on the cadF Virulence Gene and Its Product
Journal of clinical microbiology, Vol.37(3), pp.510-517
03/1999
Handle:
https://hdl.handle.net/2376/107965
PMCID: PMC84446
PMID: 9986804
Abstract
Campylobacter jejuni
and
Campylobacter coli
are common causes of gastroenteritis in humans. Infection with
C. jejuni
or
C. coli
is commonly acquired by eating undercooked chicken. The goal of this study was to develop specific detection assays for
C. jejuni
and
C. coli
isolates based on the
cadF
virulence gene and its product. The
cadF
gene from
C. jejuni
and
C. coli
encodes a 37-kDa outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells. A fragment of approximately 400 bp was amplified from 38 of 40 (95%)
C. jejuni
isolates and 5 of 6 (83.3%)
C. coli
isolates with primers designed to amplify an internal fragment of the
cadF
gene. PCR was then used to amplify
Campylobacter
DNA from store-bought chickens. A 400-bp band was amplified from 26 of the 27 chicken carcasses tested by the PCR-based assay. The CadF protein was detected in every
C. jejuni
and
C. coli
isolate tested, as judged by immunoblot analysis with a rabbit anti-
C. jejuni
37-kDa serum. In addition, methanol-fixed samples of whole-cell
C. jejuni
and
C. coli
were detected with the rabbit anti-37-kDa serum by using an indirect-immunofluorescence microscopy assay. These findings indicate that the
cadF
gene and its product are conserved among
C. jejuni
and
C. coli
isolates and that a PCR assay based on the
cadF
gene may be useful for the detection of
Campylobacter
organisms in food products.
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Details
- Title
- Identification of the Enteropathogens Campylobacter jejuni and Campylobacter coli Based on the cadF Virulence Gene and Its Product
- Creators
- Michael E Konkel - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Sean A Gray - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Bong J Kim - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Steven G Garvis - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Julie Yoon - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233
- Publication Details
- Journal of clinical microbiology, Vol.37(3), pp.510-517
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547136101842
- Language
- English
- Resource Type
- Journal article