Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas

B W Baron; G Nucifora; N McCabe; R Espinosa, 3rd; M M Le Beau; T W McKeithan

doi:10.1073/pnas.90.11.5262

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Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas

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Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas

B W Baron, G Nucifora, N McCabe, R Espinosa, 3rd, M M Le Beau and T W McKeithan

Proceedings of the National Academy of Sciences - PNAS, Vol.90(11), pp.5262-5266

06/01/1993

DOI: https://doi.org/10.1073/pnas.90.11.5262

Handle:

https://hdl.handle.net/2376/113124

PMCID: PMC46696

PMID: 8506375

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Abstract

Blotting, Southern

Translocation, Genetic

Humans

Molecular Sequence Data

Lymphoma, B-Cell - genetics

Restriction Mapping

Chromosomes, Human, Pair 3

In Situ Hybridization

Base Sequence

Karyotyping

Cloning, Molecular

Chromosomes, Human, Pair 22

Oncogenes

Oligodeoxyribonucleotides

Chromosome Banding

DNA, Neoplasm - isolation & purification

Chromosome Mapping

Immunoglobulin Joining Region - genetics

DNA - genetics

Genes, Immunoglobulin

DNA - isolation & purification

Animals

Gene Rearrangement

Chromosomes, Human, Pair 14

DNA, Neoplasm - genetics

Immunoglobulin Heavy Chains - genetics

Chromosomal translocations involving chromosome 3, band q27, are among the most common rearrangements in B-cell non-Hodgkin lymphoma. From a bacteriophage lambda library prepared from a lymphoma characterized by a t(3;14)(q27;q32), genomic clones were isolated using a probe from the immunoglobulin heavy chain locus (IGH) joining region. In addition to clones containing an apparently normal IGH rearrangement, others were found to contain one of the translocation breakpoint junctions. Normal chromosome 3 sequences and the reciprocal breakpoint junction were subsequently isolated. DNA probes on each side of the chromosome 3 breakpoint hybridized at high stringency to the DNA of various mammalian species, demonstrating evolutionary conservation. One such probe from the presumptive der(3) chromosome detected an 11-kilobase transcript when hybridized to RNA of B- and T-cell lines. A probe made from partial cDNA clones isolated from a T-cell line hybridized with genomic DNA from both sides of the chromosome 3 breakpoint, indicating that the t(3;14) is associated with a break within the gene on chromosome 3. In situ chromosomal hybridization revealed that the same gene is involved in the t(3;22)(q27;q11). Preliminary nucleotide sequencing shows no identity of the cDNA to gene sequences in available data banks. We propose the name BCL6 (B-cell lymphoma 6) for this gene, since it is likely to play a role in the pathogenesis of certain B-cell lymphomas.

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Title: Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas
Creators: B W Baron - Department of Pathology, University of Chicago, IL 60637
G Nucifora
N McCabe
R Espinosa, 3rd
M M Le Beau
T W McKeithan
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.90(11), pp.5262-5266
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: CA 55356 / NCI NIH HHS ST 32 CA09273 / OHS HRSA HHS CA 40046 / NCI NIH HHS
Identifiers: 99900547788601842
Language: English
Resource Type: Journal article