Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

C.R Wyatt; E.J Brackett; L.E Perryman; W.C Davis

doi:10.1016/0165-2427(95)05535-5

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Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

Journal article

Open access

Peer reviewed

Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

C.R Wyatt, E.J Brackett, L.E Perryman and W.C Davis

Veterinary immunology and immunopathology, Vol.52(1), pp.91-103

1996

DOI: https://doi.org/10.1016/0165-2427(95)05535-5

Handle:

https://hdl.handle.net/2376/111231

PMCID: PMC7119672

PMID: 8807779

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https://doi.org/10.1016/0165-2427(95)05535-5View

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Abstract

TcR1 + lymphocytes

Calves

Ileum

Mucosa

Ileal intraepithelial and lamina propria lymphocytes from newborn, 1.5-week-old, and 3-week-old calves were compared to determine to what extent the mucosa becomes populated after birth. Single and dual fluorescence flow cytometry were used with monoclonal antibodies to bovine (Bo) CD molecules to identify lymphocyte subpopulations. Few ileal mucosal lymphocytes were present in calves at birth. However, by 1.5 weeks of age, the villi were populated with large numbers of lymphocytes, and by 3 weeks of age, the numbers had increased further. These included a prominent subpopulation of γδT cells. Several subsets of γδT cells populated ileal mucosa after birth. The predominant subset coexpressed BoCD2, and a smaller subset coexpressed BoCD8. WC1 + γδT cells comprised the smallest subset. All γδT cell subsets coexpressed ACT2, a molecule expressed on activated WC1 + and WC1 − γδT cells from cattle.

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Title: Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth
Creators: C.R Wyatt - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
E.J Brackett - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
L.E Perryman - Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St., Raleigh, NC 27606, USA
W.C Davis - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
Publication Details: Veterinary immunology and immunopathology, Vol.52(1), pp.91-103
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: Elsevier B.V
Identifiers: 99900547152601842
Language: English
Resource Type: Journal article