Journal article
Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol–gel film for amperometric detection of organophosphorous insecticide
Biosensors & bioelectronics, Vol.23(1), pp.130-134
2007
Handle:
https://hdl.handle.net/2376/101838
PMID: 17499494
Abstract
A simple method to immobilize acetylcholinesterase (AChE) on silica sol–gel (SiSG) film assembling gold nanoparticles (AuNPs) was proposed, thus a sensitive, fast and stable amperometric sensor for quantitative determination of organophosphorous insecticide was developed. The large quantities of hydroxyl groups in the sol–gel composite provided a biocompatible microenvironment around enzyme molecule and stabilized its biological activity to a large extent. The immobilized AChE could catalyze the hydrolysis of acetylthiocholine chloride (ATCl) with a
K
m
app
value of 450
μM to form thiocholine, which was then oxidized to produce detectable single with a linear range of 10–1000
μM. AuNPs catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide on the enzymatic activity of AChE, using monocrotophos as a model compound, the conditions for detection of the insecticide were optimized. The inhibition of monocrotophos was proportional to its concentration ranging from 0.001 to 1
μg/ml and 2 to 15
μg/ml, with the correlation coefficients of 0.9930 and 0.9985, respectively. The detection limit was 0.6
ng/ml at a 10% inhibition. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors.
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Details
- Title
- Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol–gel film for amperometric detection of organophosphorous insecticide
- Creators
- Dan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR ChinaShizhen Chen - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR ChinaJie Cai - The Technology Center of Wuhan Iron & Steel Company, Wuhan 430080, PR ChinaAidong Zhang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
- Publication Details
- Biosensors & bioelectronics, Vol.23(1), pp.130-134
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900546674701842
- Language
- English
- Resource Type
- Journal article