Journal article
Immobilization of glucose oxidase into polyaniline nanofiber matrix for biofuel cell applications
Biosensors & bioelectronics, Vol.26(9), pp.3908-3913
2011
Handle:
https://hdl.handle.net/2376/114315
PMID: 21470844
Abstract
Glucose oxidase (GOx) was immobilized into the porous matrix of polyaniline nanofibers in a three-step process, consisting of enzyme adsorption, precipitation, and crosslinking (EAPC). EAPC was highly active and stable when compared to the control samples of enzyme adsorption (EA) and enzyme adsorption and crosslinking (EAC) with no step of enzyme precipitation. The GOx activity of EAPC was 9.6 and 4.2 times higher than those of EA and EAC, respectively. Under rigorous shaking at room temperature for 56
days, the relative activities of EA, EAC and EAPC, defined as the percentage of residual activity to the initial activity, were 22%, 19% and 91%, respectively. When incubated at 50
°C under shaking for 4
h, EAPC showed a negligible decrease of GOx activity while the relative activities of EA and EAC were 45% and 48%, respectively. To demonstrate the feasible application of EAPC in biofuel cells, the enzyme anodes were prepared and used for home-built air-breathing biofuel cells. The maximum power densities of biofuel cells with EA and EAPC anodes were 57 and 292
μW/cm
2, respectively. After thermal treatment at 60
°C for 4
h, the maximum power density of EA and EAPC anodes were 32 and 315
μW/cm
2, representing 56% and 108% of initially obtained maximum power densities, respectively. Because the lower power densities and short lifetime of biofuel cells are serious problems against their practical applications, the present results with EAPC anode has opened up a new potential for the realization of practical biofuel cell applications.
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Details
- Title
- Immobilization of glucose oxidase into polyaniline nanofiber matrix for biofuel cell applications
- Creators
- Hyeongseok Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaInseon Lee - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaYongchai Kwon - Graduate School of Energy and Environment, Seoul National University of Science and Technology, Seoul 139-743, Republic of KoreaByoung Chan Kim - Environment Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of KoreaSu Ha - School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, USAJung-heon Lee - Department of Chemical Engineering, Chosun University, Gwangju 501-759, Republic of KoreaJungbae Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea
- Publication Details
- Biosensors & bioelectronics, Vol.26(9), pp.3908-3913
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548252501842
- Language
- English
- Resource Type
- Journal article