Journal article
Immobilization of β-glucosidase on Eupergit C for Lignocellulose Hydrolysis
Biotechnology letters, Vol.28(3), pp.151-156
02/2006
Handle:
https://hdl.handle.net/2376/101106
PMID: 16489491
Abstract
β-Glucosidase is frequently used to supplement cellulase preparations for hydrolysis of cellulosic and lignocellulosic substrates in order to accelerate the conversion of cellobiose to glucose. Typically, commercial cellulase preparations are deficient in this enzyme and accumulation of cellobiose leads to product inhibition. This study evaluates the potential for recycling β-glucosidase by immobilization on a methacrylamide polymer carrier, Eupergit C. The immobilized β-glucosidase had improved stability at 65 °C, relative to the free enzyme, while the profile of activity versus pH was unchanged. Immobilization resulted in an increase in the apparent Km from 1.1 to 11 mm and an increase in Vmax from 296 to 2430 μmol mg−1 min−1. The effect of immobilized β-glucosidase on the hydrolysis of cellulosic and lignocellulosic substrates was comparable to that of the free enzyme when used at the same level of protein. Operational stability of the immobilized β-glucosidase was demonstrated during six rounds of lignocellulose hydrolysis.
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Details
- Title
- Immobilization of β-glucosidase on Eupergit C for Lignocellulose Hydrolysis
- Creators
- Maobing Tu - Department of Wood Science, Forest Sciences Centre University of British Columbia 2424 Main Mall V6T 1Z4 Vancouver BC CanadaXiao Zhang - Pulp and Paper Research Institute of Canada 570 Boulevard St-Jean H9R 3J9 Pointe Claire QC CanadaArwa Kurabi - Department of Wood Science, Forest Sciences Centre University of British Columbia 2424 Main Mall V6T 1Z4 Vancouver BC CanadaNeil Gilkes - Department of Wood Science, Forest Sciences Centre University of British Columbia 2424 Main Mall V6T 1Z4 Vancouver BC CanadaWarren Mabee - Department of Wood Science, Forest Sciences Centre University of British Columbia 2424 Main Mall V6T 1Z4 Vancouver BC CanadaJack Saddler - Department of Wood Science, Forest Sciences Centre University of British Columbia 2424 Main Mall V6T 1Z4 Vancouver BC Canada
- Publication Details
- Biotechnology letters, Vol.28(3), pp.151-156
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900546516101842
- Language
- English
- Resource Type
- Journal article