Journal article
Immortalization and characterization of a Sertoli cell line from the adult rat
Biology of reproduction, Vol.53(6), pp.1446-1453
12/1995
Handle:
https://hdl.handle.net/2376/116201
PMID: 8562702
Abstract
To facilitate investigations of the regulation of adult Sertoli cell function, we have established a Sertoli cell line from sexually mature Sprague-Dawley rats. The cells were immortalized with the temperature-sensitive mutant of the SV40 virus, tsA255. The tsA255 large T antigen is heat-labile and efficiently promotes propagation of cells at 33 degrees C (permissive temperature) but is inactive at 40 degrees C (nonpermissive temperature). The established clonal Sertoli cell line (ASC-17D) proliferates indefinitely at the permissive temperature. However, within 48 h at the nonpermissive temperature, cell proliferation ceases. ASC-17D cells show positive staining with antibodies to cytokeratin and vimentin, consistent with the Sertoli cell origin of these cells. Transferrin and sulfated glycoprotein (SGP)-2 mRNAs were nearly undetectable in ASC-17D cells cultured at the permissive temperature, but expression of both mRNAs was induced at the nonpermissive temperature. In contrast, SGP-1 was expressed equally at both the permissive and nonpermissive temperatures. There was no increase in either transferrin or SGP-2 with FSH or dibutyryl cAMP (db-cAMP) treatment at the permissive temperature or with FSH treatment at the nonpermissive temperature. However, the steady-state levels of both of these mRNAs were substantially increased in the presence of db-cAMP at the nonpermissive temperature. In contrast, SGP-1 mRNA was not affected by either FSH or db-cAMP. These results suggest that the ASC-17D cell line is derived from adult Sertoli cells and may be useful for the study of adult Sertoli cell function.
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Details
- Title
- Immortalization and characterization of a Sertoli cell line from the adult rat
- Creators
- K P Roberts - Department of Urologic Surgery, University of Minnesota Medical School, Minneapolis 55455, USA. roberts@lenti.med.umn.eduP P BanerjeeJ W TindallB R Zirkin
- Publication Details
- Biology of reproduction, Vol.53(6), pp.1446-1453
- Academic Unit
- Elson S. Floyd College of Medicine
- Publisher
- United States
- Grant note
- HD09921 / NICHD NIH HHS
- Identifiers
- 99900548301501842
- Language
- English
- Resource Type
- Journal article