Journal article
Immunization of BALB/c Mice with Killed Neospora caninum Tachyzoite Antigen Induces a Type 2 Immune Response and Exacerbates Encephalitis and Neurological Disease
Clinical and diagnostic laboratory immunology, Vol.7(6), pp.893-898
11/2000
Handle:
https://hdl.handle.net/2376/118378
PMCID: PMC95981
PMID: 11063494
Abstract
BALB/c mice were immunized subcutaneously with soluble
Neospora caninum
tachyzoite antigen (NSO) entrapped in nonionic surfactant vesicles (NISVs) or administered with Freund's complete adjuvant (FCA). Following virulent parasite challenge, groups of mice immunized with NSO and either NISVs or FCA had clinical neurological disease and increased numbers of brain lesions compared to groups of mice inoculated with FCA, NISVs, or phosphate-buffered saline (PBS) alone. Increased numbers of brain lesions were statistically significant only between mice immunized with NISV-NSO and NISV- or PBS-treated mice. Following parasite challenge, brain inflammatory infiltrates in all experimental and control groups of mice were relatively similar and consisted of compact infiltrates of macrophages admixed with various numbers of lymphoid cells. Increased brain lesions in NSO-immunized mice were associated with increased antigen-specific interleukin 4 (IL-4) secretion and increased IL-4:gamma interferon secretion ratios from splenocytes in vitro and increased antigen-specific immunoglobulin G1 (IgG1):IgG2a ratios in vivo. Thus, immunization with whole killed
N. caninum
antigen and either liposoidal or Freund's adjuvant induced a type 2 immune response that was associated with worsened disease. The present studies emphasize the need to identify specific
N. caninum
antigens or other delivery systems that will elicit protective immune responses to neosporosis.
Metrics
7 Record Views
Details
- Title
- Immunization of BALB/c Mice with Killed Neospora caninum Tachyzoite Antigen Induces a Type 2 Immune Response and Exacerbates Encephalitis and Neurological Disease
- Creators
- Timothy V Baszler - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonTerry F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonBruce A Mathison - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.7(6), pp.893-898
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547823901842
- Language
- English
- Resource Type
- Journal article