Journal article
Immunization of cattle with a 36-kilodalton surface protein induces protection against homologous and heterologous Anaplasma marginale challenge
Infection and immunity, Vol.56(6), pp.1526-1531
06/1988
Handle:
https://hdl.handle.net/2376/105109
PMCID: PMC259431
PMID: 3372018
Abstract
Immunization of cattle with a purified Anaplasma marginale major surface protein, AmF36, induced protection against homologous challenge with the Florida isolate. Similarly, immunized cattle were protected from challenge with the antigenically and structurally distinct Washington-O isolate of A. marginale. The degree of protection in AmF36-immunized cattle varied from complete prevention of rickettsemia to significant delay in the onset of rickettsemia compared with control immunized cattle. A single AmF36 vaccinate was not protected against homologous challenge despite development of a strong antibody response. Immunoprecipitation of A. marginale proteins with a monoclonal antibody to AmF36 identified minor molecular size heterogeneity in this protein from different isolates, including the Florida and Washington-O isolates. The apparent molecular size of this surface protein in the Florida isolate was 36 kilodaltons, whereas the analogous proteins in Washington-O and four other isolates of A. marginale from the United States had molecular masses of 33 to 34 kilodaltons. Significantly, the surface-exposed peptides of these proteins appear to be conserved among the different isolates. These results demonstrate the potential of AmF36 as a subunit immunogen for bovine anaplasmosis and indicate a structural basis for its cross-protective ability.
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Details
- Title
- Immunization of cattle with a 36-kilodalton surface protein induces protection against homologous and heterologous Anaplasma marginale challenge
- Creators
- G H Palmer - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610S M Oberle - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610A F Barbet - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610W L Goff - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610W C Davis - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610T C McGuire - Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610
- Publication Details
- Infection and immunity, Vol.56(6), pp.1526-1531
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546734601842
- Language
- English
- Resource Type
- Journal article