Journal article
Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice
International journal for parasitology, Vol.35(13), pp.1407-1415
2005
Handle:
https://hdl.handle.net/2376/101267
PMID: 16051244
Abstract
NcSRS2, a tachyzoite surface protein of
Neospora caninum, is an immunodominant protein with respect to induction of antibody production and has a role in attachment and invasion of host cells. Native NcSRS2 was isolated from whole tachyzoite lysate antigen by affinity chromatography using NcSRS2 specific monoclonal antibody and used to immunize BALB/c mice in a congenital transmission study. NcSRS2 was a highly conserved protein as indicated by comparison of deduced amino acid sequence obtained from NcSRS2 gene sequences of 10 geographically distinct
N. caninum isolates. Mice immunized with purified native NcSRS2 produced antigen-specific antibody, primarily of IgG 1 subtype. Following challenge during gestation with 10
7 tachyzoites, immunized mice had a statistically significant decreased frequency of congenital transmission compared to non-immunized mice (
P≤0.05) or mice inoculated with adjuvant alone (
P≤0.01). Decreased congenital transmission among immunized mice correlated with a predominately Th2 immune response compared to non-immunized mice as indicated by an increased ratio of interleukin 4 (IL-4) to interferon gamma (IFN-γ) secretion from antigen-stimulated splenocytes. The results provide a rationale for NcSRS2 as a candidate subunit vaccine antigen for reduction of
N. caninum congenital transmission. Furthermore, the studies suggest that a Th2 immune response, if directed against an appropriate antigen, may induce protection against
N. caninum congenital infection in mice.
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Details
- Title
- Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice
- Creators
- G.J Haldorson - Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Pullman, WA 99164-7040, USAB.A Mathison - Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Pullman, WA 99164-7040, USAK Wenberg - Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Pullman, WA 99164-7040, USAP.A Conrad - Department of Veterinary Microbiology and Immunology, University of California, Davis, CA 95616, USAJ.P Dubey - Animal Parasitic Disease Laboratory, United States Department of Agriculture, Agricultural Research Services, Beltsville, MD 20705-2350, USAA.J Trees - Veterinary Parasitology, Liverpool School of Tropical Medicine and Faculty of Veterinary Science, University of Liverpool, Liverpool L35QA, UKI Yamane - National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba-shi, Ibaraki Japan 305T.V Baszler - Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Pullman, WA 99164-7040, USA
- Publication Details
- International journal for parasitology, Vol.35(13), pp.1407-1415
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546643901842
- Language
- English
- Resource Type
- Journal article