Journal article
Immunodiagnostic test for detection of serum antibody to Treponema pallidum (Syphilis): Fibronectin as a capture vehicle for treponemal adhesins
Journal of immunological methods, Vol.84(1), pp.365-373
1985
Handle:
https://hdl.handle.net/2376/105926
PMID: 3905972
Abstract
Knowledge that
Treponema pallidum adhesin proteins bind to host fibronectin (Fn) via ligand-receptor interactions has resulted in development of an ELISA for measuring specific antitreponemal antibodies in sera of syphilitic patients and infected experimental animals. As little as 50 ng of
T. pallidum total protein extract added to Fn-coated wells permitted half-maximal levels of ELISA reactivity. Detection of serum antibody from intratesticularly infected rabbits occured at dilutions greater than 1/100,000. Antibody titers in serum from patients with primary and latent syphilis were positive at 1/1000 dilutions while serum samples from patients with secondary syphilis were reactive at 1/10,000. Furthermore, the ELISA proved useful for evaluating serum samples from individuals with other treponemal infections. Antibodies raised against the non-pathogenic spirochete,
T. phagedenis biotype Reiter, were non-reactive with Fn-
T. palladum complexes. Also, Reiter treponemal proteins did not bind to Fn-coated wells. This ELISA using Fn as a capture vehicle for treponemal adhesin proteins was superior to 3 other routinely used tests for syphilis diagnosis.
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Details
- Title
- Immunodiagnostic test for detection of serum antibody to Treponema pallidum (Syphilis): Fibronectin as a capture vehicle for treponemal adhesins
- Creators
- John F AldereteLinda Freeman-ShadeJoel B Baseman
- Publication Details
- Journal of immunological methods, Vol.84(1), pp.365-373
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546618801842
- Language
- English
- Resource Type
- Journal article