Journal article
Immunogenic B-cell epitopes of Babesia bovis rhoptry-associated protein 1 are distinct from sequences conserved between species
Infection and immunity, Vol.61(8), pp.3511-3517
08/1993
Handle:
https://hdl.handle.net/2376/108975
PMCID: PMC281030
PMID: 7687587
Abstract
Babesia bovis merozoite apical membrane polypeptide Bv60 was found to be rhoptry associated by immuno-electron microscopy and was redesignated rhoptry-associated protein 1 (RAP-1). The N-terminal 300 amino acids of RAP-1 have a high level of sequence similarity to the same N-terminal region of p58, its homolog from Babesia bigemina. However, the interspecies conserved sequences did not include RAP-1 surface-exposed B-cell epitopes as defined by monoclonal antibodies. Furthermore, neither heterologous B. bigemina immune nor monospecific anti-p58 bovine serum binds to whole RAP-1, indicating that the major B-cell epitopes recognized by these sera are also not encoded by the conserved sequences. Truncated RAP-1, expressed by a subclone encoding the N-terminal 235 amino acids, is weakly bound by antibodies in heterologous sera. A peptide representing the longest conserved amino acid sequence (amino acids 121 to 134) in this region is also weakly bound by antibodies in immune bovine sera, and rabbit antibodies induced by and strongly reactive with the peptide alone fail to bind native or denatured RAP-1. Thus, although the conserved region may contain one or more poorly immunogenic B-cell epitopes, these epitopes are inaccessible to antibody in whole RAP-1. The results indicate that the major immunogenic B-cell epitopes of RAP-1, including surface-accessible epitopes bound by monoclonal antibodies, are distinct from the conserved sequences representing putative functional domains.
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Details
- Title
- Immunogenic B-cell epitopes of Babesia bovis rhoptry-associated protein 1 are distinct from sequences conserved between species
- Creators
- C E Suarez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040G H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040S A Hines - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040T F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040
- Publication Details
- Infection and immunity, Vol.61(8), pp.3511-3517
- Academic Unit
- Veterinary Medicine, College of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900547123001842
- Language
- English
- Resource Type
- Journal article