Journal article
Immunohistochemical Evaluation of Canine Pituitary Adenomas Obtained by Transsphenoidal Hypophysectomy
Veterinary pathology, Vol.55(6), pp.889-895
11/2018
Handle:
https://hdl.handle.net/2376/109930
PMID: 29925292
Abstract
Hypophysectomy specimens from 16 dogs with pituitary adenoma were evaluated with periodic acid-Schiff (PAS), reticulin, and immunohistochemistry for adrenocorticotrophic hormone (ACTH), melanocyte stimulating hormone (MSH), growth hormone (GH), and Ki-67. The reticulin network was obliterated in all adenomas. One adenoma expressed ACTH and GH. Eight corticotroph adenomas were basophilic to chromophobic, and PAS- and ACTH-positive. Seven melanotroph adenomas were distinguished from corticotroph adenomas by expression of MSH. Pituitary-dependent hypercortisolism was diagnosed in 5 of 8 dogs with corticotroph and 4 of 7 with melanotroph adenoma. Pituitary height/brain area (P/B) ratio was elevated in all dogs. Previous canine hypophysectomy studies suggested that melanotroph adenomas were larger and carried a worse prognosis than corticotroph adenomas; however, in this study, corticotroph adenomas in comparison to melanotroph adenomas were larger (median P/B ratio: 1.06 versus 0.76), more proliferative (median Ki-67 index: 9.47% versus 1.99%), and associated with shorter survival (median: 300 versus 793 days). Recommended immunohistochemistry for PAS-positive pituitary adenomas includes ACTH and MSH to distinguish corticotrophs from melanotrophs and Ki-67 for proliferation index.
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Details
- Title
- Immunohistochemical Evaluation of Canine Pituitary Adenomas Obtained by Transsphenoidal Hypophysectomy
- Creators
- Margaret A Miller - 2 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USATina Jo Owen - 3 Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, Washington State University, Pullman, WA, USADavid S Bruyette - 4 Anivive Life Sciences, Long Beach, CA, USAJ Catharine Scott-Moncrieff - 5 Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, Purdue University, West Lafayette, IN, USAJosé A Ramos-Vara - 2 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USAHsin-Yi Weng - 2 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USAAnnie V Chen - 3 Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, Washington State University, Pullman, WA, USALinda G Martin - 3 Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, Washington State University, Pullman, WA, USADeidre M DuSold - 1 Indiana Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN, USA
- Publication Details
- Veterinary pathology, Vol.55(6), pp.889-895
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900547298301842
- Language
- English
- Resource Type
- Journal article