Journal article
Immunological assay for carbohydrate antigen 19-9 using an electrochemical immunosensor and antigen immobilization in titania sol–gel matrix
Journal of immunological methods, Vol.283(1), pp.67-75
2003
Handle:
https://hdl.handle.net/2376/109454
PMID: 14659900
Abstract
We describe a novel electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) based on the immobilization of CA19-9 with titania sol–gel on a graphite electrode (GE) by vapor deposition. The CA19-9 membrane was characterized using scanning electron microscopy and proved to be chemically clean, porous and homogeneous. The incubation of the immunosensor in a solution containing horseradish peroxidase (HRP)-labeled CA19-9 antibody led to the binding of HRP-labeled antibody with the immobilized antigen. The immobilized HRP catalyzed the oxidation of catechol by H
2O
2 and this provided a competitive method for the measurement of serum CA19-9. The response current decreased with increasing CA19-9 concentration in the incubation solution. The effects of pH, amount of HRP-labeled antibody, incubation time and temperature were explored to provide optimum analytical performance. Under optimal conditions, the current decrease of the immunosensor was proportional to CA19-9 concentrations in the range of 3–20 U/ml with a detection limit of 2.68 U/ml at a current decrease of 10%. The detection of CA19-9 in two serum samples obtained from clinically diagnosed patients with pancreatic carcinoma showed acceptable accuracy. The proposed immunosensor provides a new promising tool for the clinical immunoassay of CA19-9.
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Details
- Title
- Immunological assay for carbohydrate antigen 19-9 using an electrochemical immunosensor and antigen immobilization in titania sol–gel matrix
- Creators
- Dan Du - State Key Laboratory of Coordination Chemistry, Department of Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, PR ChinaFeng Yan - Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009, PR ChinaShengli Liu - The Affiliated Zhongda Hospital, Southeast University, Nanjing 210009, PR ChinaHuangxian Ju - State Key Laboratory of Coordination Chemistry, Department of Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, PR China
- Publication Details
- Journal of immunological methods, Vol.283(1), pp.67-75
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547260501842
- Language
- English
- Resource Type
- Journal article