Journal article
Immunopathology of a two-hit murine model of acid aspiration lung injury
American journal of physiology. Lung cellular and molecular physiology, Vol.278(3), pp.L512-L520
03/01/2000
Handle:
https://hdl.handle.net/2376/110533
PMID: 10710523
Abstract
In a two-hit model of acid aspiration lung injury, mice were subjected to nonlethal cecal ligation and puncture (CLP). After 48 h, intratracheal (IT) acid was administered, and mice were killed at several time points. Recruitment of neutrophils in response to acid was documented by myeloperoxidase assay and neutrophil counts in bronchoalveolar lavage (BAL) fluid and peaked at 8 h post-IT injection. Albumin in BAL fluid, an indicator of lung injury, also peaked at 8 h. When the contributions of the two hits were compared, neutrophil recruitment and lung injury occurred in response to acid but were not greatly influenced by addition of another hit. Neutrophil sequestration was preceded by elevations in KC and macrophage inflammatory protein-2α in plasma and BAL fluid. KC levels in BAL fluid were higher and peaked earlier than macrophage inflammatory protein-2α levels. When KC was blocked with specific antiserum, neutrophil recruitment was significantly reduced, whereas albumin in BAL fluid was not affected. In conclusion, murine KC mediated neutrophil recruitment but not lung injury in a two-hit model of aspiration lung injury.
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Details
- Title
- Immunopathology of a two-hit murine model of acid aspiration lung injury
- Creators
- J. A Nemzek - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602D. R Call - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602S. J Ebong - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602D. E Newcomb - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602G. L Bolgos - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602D. G Remick - Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109-0602
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.278(3), pp.L512-L520
- Identifiers
- 99900547672801842
- Language
- English
- Resource Type
- Journal article