Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege

Timothy J Doyle; Gurvinder Kaur; Saroja M Putrevu; Emily L Dyson; Mathew Dyson; William T McCunniff; Mithun R Pasham; Kwan Hee Kim; Jannette M Dufour

doi:10.1095/biolreprod.110.089425

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Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege

Journal article

Open access

Peer reviewed

Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege

Timothy J Doyle, Gurvinder Kaur, Saroja M Putrevu, Emily L Dyson, Mathew Dyson, William T McCunniff, Mithun R Pasham, Kwan Hee Kim and Jannette M Dufour

Biology of reproduction, Vol.86(1), pp.1-14

01/2012

DOI: https://doi.org/10.1095/biolreprod.110.089425

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https://hdl.handle.net/2376/104515

PMID: 21900683

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Abstract

Cell Line

Sertoli Cells - transplantation

Gene Expression Regulation - immunology

Mice, Inbred C57BL

Male

Gene Expression Profiling

Reverse Transcriptase Polymerase Chain Reaction

Cell Adhesion

Animals

Sertoli Cells - immunology

Tight Junctions

Adherens Junctions

Mice

Mice, Inbred BALB C

Real-Time Polymerase Chain Reaction

Apoptosis

Primary Sertoli cells isolated from mouse testes survive when transplanted across immunological barriers and protect cotransplanted allogeneic and xenogeneic cells from rejection in rodent models. In contrast, the mouse Sertoli cell line (MSC-1) lacks immunoprotective properties associated with primary Sertoli cells. In this study, enriched primary Sertoli cells or MSC-1 cells were transplanted as allografts into the renal subcapsular area of naive BALB/c mice, and their survival in graft sites was compared. While Sertoli cells were detected within the grafts with 100% graft survival throughout the 20-day study, MSC-1 cells were rejected between 11 and 14 days, with 0% graft survival at 20 days posttransplantation. Nonetheless, the mechanism for primary Sertoli cell survival and immunoprotection remains unresolved. To identify immune factors or functional pathways potentially responsible for immune privilege, gene expression profiles of enriched primary Sertoli cells were compared with those of MSC-1 cells. Microarray analysis identified 2369 genes in enriched primary Sertoli cells that were differentially expressed at ±4-fold or higher levels than in MSC-1 cells. Ontological analyses identified multiple immune pathways, which were used to generate a list of 340 immune-related genes. Three functions were identified in primary Sertoli cells as potentially important for establishing immune privilege: suppression of inflammation by specific cytokines and prostanoid molecules, slowing of leukocyte migration by controlled cell junctions and actin polymerization, and inhibition of complement activation and membrane-associated cell lysis. These results increase our understanding of testicular immune privilege and, in the long-term, could lead to improvements in transplantation success.

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Title: Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege
Creators: Timothy J Doyle - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, USA
Gurvinder Kaur
Saroja M Putrevu
Emily L Dyson
Mathew Dyson
William T McCunniff
Mithun R Pasham
Kwan Hee Kim
Jannette M Dufour
Publication Details: Biology of reproduction, Vol.86(1), pp.1-14
Publisher: United States
Grant note: R01 HD067400 / NICHD NIH HHS HD067400 / NICHD NIH HHS
Identifiers: 99900546955101842
Language: English
Resource Type: Journal article