Journal article
In Vivo Binding of Immunoglobulin M to the Surfaces of Babesia bigemina-Infected Erythrocytes
Infection and immunity, Vol.66(6), pp.2922-2927
06/1998
Handle:
https://hdl.handle.net/2376/100399
PMCID: PMC108290
PMID: 9596768
Abstract
Babesia bigemina
infection of mature bovine erythrocytes results in new proteins specifically exposed on the parasitized cell surface. Monoclonal antibody (MAb) 64/32 binds a protein, designated p94, on
B. bigemina
-infected erythrocytes but not on either uninfected or
B. bovis
-parasitized erythrocytes. However, p94 was not encoded by
B. bigemina
and was not a parasite-modified erythrocyte membrane protein. In contrast, we showed that p94 could be eluted from the infected erythrocyte surface and was identified as specifically bound immunoglobulin M (IgM) heavy chain for the following reasons: (i) MAb 64/32 bound a reduced molecule of 94 kDa in both infected erythrocyte lysates and normal bovine serum; (ii) MAb 64/32 bound a 94-kDa molecule in reduced preparations of purified IgM; (iii) an anti-bovine μ heavy-chain MAb, BIg73, reacted specifically with the surface of infected erythrocytes and bound the 94-kDa molecule in lysates of infected erythrocytes, normal bovine serum, and purified IgM; and (iv) immunoprecipitation of infected erythrocyte lysates with MAb 64/32 depleted the 94-kDa antigen bound by anti-μ MAb BIg73 and vice versa. Binding of IgM to the infected erythrocyte surface was detected in vivo early in acute parasitemia and occurred during both the trophozoite and merozoite stages of intraerythrocytic parasitism. The common feature of IgM binding to the parasitized erythrocyte surface among otherwise genetically and antigenically distinct
B. bigemina
strains is suggestive of an advantageous role in parasite survival in vivo.
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Details
- Title
- In Vivo Binding of Immunoglobulin M to the Surfaces of Babesia bigemina-Infected Erythrocytes
- Creators
- Ignacio E Echaide - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164Stephen A Hines - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164Terry F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164Carlos E Suarez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164Travis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164Guy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164
- Publication Details
- Infection and immunity, Vol.66(6), pp.2922-2927
- Academic Unit
- Veterinary Medicine, College of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546685501842
- Language
- English
- Resource Type
- Journal article