Journal article
In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design
Talanta (Oxford), Vol.74(5), pp.1337-1343
2008
Handle:
https://hdl.handle.net/2376/103851
PMID: 18371787
Abstract
Gold nanoparticles (AuNPs) were synthesized in situ and electrodeposited onto Au substrate. The AuNPs modified interface facilitates electron transfer across self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA). After activation of surface carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, the interface displayed good stability for immobilization of biomolecules. These modification processes were characterized by contact angle measurement, cyclic voltammetry and electrochemical impedance spectra. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate, leading to a stable AChE biosensor. Under the optimal experimental conditions, the inhibition of malathion on AChE biosensor was proportional to its concentration in two ranges, from 0.001 to 0.1 μg mL −1 and from 0.1 to 25 μg mL −1, with detection limit of 0.001 μg mL −1. The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design.
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Details
- Title
- In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design
- Creators
- Dan Du - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaJiawang Ding - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaJie Cai - The Technology Center of Wuhan Iron & Steel Company, Wuhan 430080, PR ChinaJianming Zhang - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaLi Liu - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
- Publication Details
- Talanta (Oxford), Vol.74(5), pp.1337-1343
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900546872701842
- Language
- English
- Resource Type
- Journal article