Journal article
In vitro and in vivo pathogenicity of Salmonella enteritidis clinical strains isolated from North America
Archives of microbiology, Vol.193(11), pp.811-821
11/2011
Handle:
https://hdl.handle.net/2376/107321
PMID: 21655947
Abstract
Salmonella enteritidis is a leading cause of food-borne gastroenteritis worldwide. In this study, 48 strains of S. enteritidis isolated from clinical cases of salmonellosis in North America were tested for their virulence-associated traits including cell invasiveness, biofilm, motility, presence of a virulence plasmid, and virulence in orally challenged mice. The majority of strains exhibited high invasiveness (n = 45), whereas only few strains (n = 3) exhibited low invasiveness. All low-invasive strains (100%, 3/3) were biofilm negative, whereas the distribution of biofilm positive and negative phenotypes among high-invasive strains was 53.4% (24/45) and 46.6% (21/45), respectively. The in vitro cell invasiveness was not associated with biofilm formation (Fisher’s exact test, P = 0.23) or the presence of a spvB gene, a marker for the virulence-associated plasmid (Fisher’s exact test, P = 1). There was no correlation between cell invasiveness and motility (Spearman’s rank test, r = −0.15; P = 0.27). Virulence testing in orally challenged mice revealed that the low-invasive strains were as virulent as high-invasive strains, indicating that in vitro cell invasiveness did not correlate with in vivo virulence. In conclusion, we show that despite phenotypic diversity among clinical strains of S. enteritidis, the majority of strains are highly invasive in vitro and in vivo.
Metrics
5 Record Views
Details
- Title
- In vitro and in vivo pathogenicity of Salmonella enteritidis clinical strains isolated from North America
- Creators
- Devendra Shah - Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USAXiaohui Zhou - Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USATarek Addwebi - Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USAMargaret Davis - Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USADouglas Call - Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USA
- Publication Details
- Archives of microbiology, Vol.193(11), pp.811-821
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547298101842
- Language
- English
- Resource Type
- Journal article