Journal article
In vitro production of llama ( Lama glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions
Animal reproduction science, Vol.99(3), pp.342-353
2007
Handle:
https://hdl.handle.net/2376/109154
PMID: 16846701
Abstract
Assisted reproductive technologies in the llama (
Lama glama) are needed to provide alternative methods for the propagation, selection and genetic improvement; however, recovery of adequate quantity and quality of spermatozoa for conventional IVF is problematic. Therefore, an effort was made to adapt the intracytoplasmic sperm injection (ICSI) procedure for the
in vitro production of llama embryos. The specific objectives of this study were: (1) to determine
in vitro maturation rates of oocytes recovered by transvaginal ultrasound-guided oocyte aspiration (TUGA) or flank laparotomy; (2) to evaluate the effects of activation treatments following ICSI; (3) to evaluate the development of llama ICSI embryos in CR1aa medium or in an oviduct cell co-culture system. Llamas were superstimulated by double dominant follicle reduction followed by oFSH administered in daily descending doses over a 3-day interval. Oocytes were harvested by flank laparotomy or TUGA and matured
in vitro for 30
h. Mature oocytes were subjected to ICSI followed by no chemical activation (Treatment A), ionomycin only (Treatment B) or ionomycin/DMAP activation (Treatment C). More oocytes were recovered by flank laparotomy procedure compared with TUGA (94% versus 61%,
P
<
0.05) and a greater number of oocytes harvested by flank laparotomy reached the metaphase-II stage (77% versus 44%,
P
<
0.05). After ICSI, the proportion of cleaved and 4–8-cell stages embryos was significantly greater when injected oocytes were activated with ionomycin/DMAP combination (63% and 38%, respectively,
P
<
0.05). The co-culture of ICSI embryos with llama oviduct epithelial cells resulted in progression to morula (25%) and blastocyst (12%) stages; whereas, all embryos cultured in CR1aa medium arrested at the 8–16-cell developmental stage.
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Details
- Title
- In vitro production of llama ( Lama glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions
- Creators
- M.J Sansinena - Embryo Biotechnology Laboratory, Reproductive Biology Center, LSU Agricultural Center, St. Gabriel, LA 70776, USAS.A Taylor - GeneSearch Inc., Bozeman, MT 59715, USAP.J Taylor - GeneSearch Inc., Bozeman, MT 59715, USAE.E Schmidt - Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USAR.S Denniston - Embryo Biotechnology Laboratory, Reproductive Biology Center, LSU Agricultural Center, St. Gabriel, LA 70776, USAR.A Godke - Embryo Biotechnology Laboratory, Reproductive Biology Center, LSU Agricultural Center, St. Gabriel, LA 70776, USA
- Publication Details
- Animal reproduction science, Vol.99(3), pp.342-353
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier B.V
- Identifiers
- 99900547023201842
- Language
- English
- Resource Type
- Journal article