In vitro studies on the colonization of bovine colonic mucosa by Shiga-toxigenic Escherichia coli (STEC)

R N Cobbold; P M Desmarchelier

doi:10.1017/S0950268803001432

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In vitro studies on the colonization of bovine colonic mucosa by Shiga-toxigenic Escherichia coli (STEC)

Journal article

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In vitro studies on the colonization of bovine colonic mucosa by Shiga-toxigenic Escherichia coli (STEC)

R N Cobbold and P M Desmarchelier

Epidemiology and infection, Vol.132(1), pp.87-94

01/2004

DOI: https://doi.org/10.1017/S0950268803001432

Handle:

https://hdl.handle.net/2376/103849

PMCID: PMC2870082

PMID: 14979594

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Abstract

Age Factors

Colony Count, Microbial

Cattle

Animals, Suckling

Dietary Fiber

Edible Grain

Escherichia coli Infections - veterinary

Colon

Fluorescent Antibody Technique, Indirect

Organ Culture Techniques

Serotyping

Cattle Diseases - microbiology

Fasting

Escherichia coli Infections - microbiology

Linear Models

Cattle Diseases - pathology

Intestinal Mucosa - microbiology

Shiga Toxins

Bacterial Adhesion

Escherichia coli O157 - classification

Animals

Analysis of Variance

Escherichia coli Infections - pathology

Biopsy

Animal Feed

Hydrogen-Ion Concentration

Intestinal Mucosa - pathology

This study investigated host-related factors that influence intestinal colonization by Shiga-toxigenic E. coli (STEC). A quantitative colonization assay was developed to comparatively measure attachment of STEC to bovine colonic tissues maintained in vitro. No differences were determined in colonization susceptibility between tissues derived from weaning calves and adult cattle, or for tissues from cattle fed grain and forage-based rations. Substrate conditions designed to represent various intra-enteric environments were tested for their effect on STEC/mucosal interaction. Under conditions corresponding to a well-fed ruminant (high volatile fatty acid and lactate concentrations, low pH), significantly less STEC colonized the mucosal surface of colonic biopsies. These results may help explain why fasted, poorly or intermittently fed cattle and pre-ruminant calves excrete STEC to a greater degree. Studies on the ecology of STEC within the ruminant gut help identify mechanisms to reduce their threat to public health.

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Title: In vitro studies on the colonization of bovine colonic mucosa by Shiga-toxigenic Escherichia coli (STEC)
Creators: R N Cobbold - Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland 4072, Australia
P M Desmarchelier
Publication Details: Epidemiology and infection, Vol.132(1), pp.87-94
Publisher: England
Identifiers: 99900546931901842
Language: English
Resource Type: Journal article