Journal article
In vivo localization of iris yellow spot tospovirus (Bunyaviridae)-encoded proteins and identification of interacting regions of nucleocapsid and movement proteins
PloS one, Vol.10(3), pp.e0118973-e0118973
2015
Handle:
https://hdl.handle.net/2376/110830
PMCID: PMC4363525
PMID: 25781476
Abstract
Localization and interaction studies of viral proteins provide important information about their replication in their host plants. Tospoviruses (Family Bunyaviridae) are economically important viruses affecting numerous field and horticultural crops. Iris yellow spot virus (IYSV), one of the tospoviruses, has recently emerged as an important viral pathogen of Allium spp. in many parts of the world. We studied the in vivo localization and interaction patterns of the IYSV proteins in uninfected and infected Nicotiana benthamiana and identified the interacting partners.
Bimolecular fluorescence complementation (BiFC) analysis demonstrated homotypic and heterotypic interactions between IYSV nucleocapsid (N) and movement (NSm) proteins. These interactions were further confirmed by pull-down assays. Additionally, interacting regions of IYSV N and NSm were identified by the yeast-2-hybrid system and β-galactosidase assay. The N protein self-association was found to be mediated through the N- and C-terminal regions making head to tail interaction. Self-interaction of IYSV NSm was shown to occur through multiple interacting regions. In yeast-2-hybrid assay, the N- and C-terminal regions of IYSV N protein interacted with an N-terminal region of IYSV NSm protein.
Our studies provide new insights into localization and interactions of IYSV N and NSm proteins. Molecular basis of these interactions was studied and is discussed in the context of tospovirus assembly, replication, and infection processes.
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Details
- Title
- In vivo localization of iris yellow spot tospovirus (Bunyaviridae)-encoded proteins and identification of interacting regions of nucleocapsid and movement proteins
- Creators
- Diwaker Tripathi - Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of AmericaGaurav Raikhy - Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of AmericaMichael M Goodin - Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of AmericaRalf G Dietzgen - QAAFI, The University of Queensland, St. Lucia, Queensland, AustraliaHanu R Pappu - Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of America
- Publication Details
- PloS one, Vol.10(3), pp.e0118973-e0118973
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900547147701842
- Language
- English
- Resource Type
- Journal article