Journal article
Increase of prolactin mRNA in the rat hypothalamus after intracerebroventricular injection of VIP or PACAP
Brain research, Vol.660(2), pp.301-308
1994
Handle:
https://hdl.handle.net/2376/112939
PMID: 7820699
Abstract
Vasoactive intestinal peptide (VIP), the structurally homologous pituitary adenylate cyclase-activating peptide (PACAP) and the pituitary hormone, prolactin (PRL) enhance rapid eye movement sleep (REMS). VIP and PACAP are both inducers of PRL gene expression and release in the pituitary gland. Little is known about PRL regulation in the brain although it is hypothesized that the REMS-promoting activity of i.c.v. administered VIP may be mediated via the activation of cerebral PRL. To test whether VIP or PACAP in fact increase intracerebral mRNA, the peptides (VIP: 30 or 300 pmol; PACAP: 220 pmol) were injected i.c.v. into rats at dark onset. 1 h later, cDNA was synthesized from purified hypothalamic mRNA. Standardized amounts were analysed for PRL using the polymerase chain reaction followed by Southern blotting and hybridization. Compared with β-actin mRNA levels, both VIP and PACAP increased PRL mRNA levels in a dose-dependent fashion though VIP was more effective on a molar basis. The previously reported alternatively spliced PRL mRNA (lacking exon 4) was not detected. The data support the hypothesis that the REMS-promoting activity of central VIP and PACAP might be mediated by cerebral PRL.
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Details
- Title
- Increase of prolactin mRNA in the rat hypothalamus after intracerebroventricular injection of VIP or PACAP
- Creators
- S Bredow - Department of Physiology and Biophysics, University of Tennessee at Memphis, 894 Union Avenue, Room 320 (Nash Building), Memphis, TN 38163, USAB Kacsóh - Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, GA 31207, USAF Obál - Department of Physiology and Biophysics, University of Tennessee at Memphis, 894 Union Avenue, Room 320 (Nash Building), Memphis, TN 38163, USAJ Fang - Department of Physiology and Biophysics, University of Tennessee at Memphis, 894 Union Avenue, Room 320 (Nash Building), Memphis, TN 38163, USAJ.M Krueger - Department of Physiology and Biophysics, University of Tennessee at Memphis, 894 Union Avenue, Room 320 (Nash Building), Memphis, TN 38163, USA
- Publication Details
- Brain research, Vol.660(2), pp.301-308
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547320601842
- Language
- English
- Resource Type
- Journal article