Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar 'Zak'

Elizabeth C Schramm; Sven K Nelson; Kimberlee K Kidwell; Camille M Steber

doi:10.1007/s00122-012-2018-0

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Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar 'Zak'

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Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar 'Zak'

Elizabeth C Schramm, Sven K Nelson, Kimberlee K Kidwell and Camille M Steber

Theoretical and applied genetics, Vol.126(3), pp.791-803

03/2013

DOI: https://doi.org/10.1007/s00122-012-2018-0

Handle:

https://hdl.handle.net/2376/108639

PMCID: PMC4241963

PMID: 23212773

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https://doi.org/10.1007/s00122-012-2018-0View

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Abstract

Dose-Response Relationship, Drug

Triticum - genetics

Abscisic Acid - pharmacology

Phenotype

Seeds - drug effects

Seeds - genetics

Genotype

Mutation

Plant Growth Regulators - pharmacology

Plant Dormancy - drug effects

As a strategy to increase the seed dormancy of soft white wheat, mutants with increased sensitivity to the plant hormone abscisic acid (ABA) were identified in mutagenized grain of soft white spring wheat "Zak". Lack of seed dormancy is correlated with increased susceptibility to preharvest sprouting in wheat, especially those cultivars with white kernels. ABA induces seed dormancy during embryo maturation and inhibits the germination of mature grain. Three mutant lines called Zak ERA8, Zak ERA19A, and Zak ERA19B (Zak ENHANCED RESPONSE to ABA) were recovered based on failure to germinate on 5 μM ABA. All three mutants resulted in increased ABA sensitivity over a wide range of concentrations such that a phenotype can be detected at very low ABA concentrations. Wheat loses sensitivity to ABA inhibition of germination with extended periods of dry after-ripening. All three mutants recovered required more time to after-ripen sufficiently to germinate in the absence of ABA and to lose sensitivity to 5 μM ABA. However, an increase in ABA sensitivity could be detected after as long as 3 years of after-ripening using high ABA concentrations. The Zak ERA8 line showed the strongest phenotype and segregated as a single semi-dominant mutation. This mutation resulted in no obvious decrease in yield and is a good candidate gene for breeding preharvest sprouting tolerance.

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Title: Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar 'Zak'
Creators: Elizabeth C Schramm - Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA
Sven K Nelson
Kimberlee K Kidwell
Camille M Steber
Publication Details: Theoretical and applied genetics, Vol.126(3), pp.791-803
Academic Unit: Crop and Soil Sciences, Department of
Publisher: Germany
Grant note: T32 GM008336 / NIGMS NIH HHS
Identifiers: 99900547186001842
Language: English
Resource Type: Journal article