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CC BY V4.0, Open Access
Journal article
Induction of ovarian primordial follicle assembly by connective tissue growth factor CTGF
PloS one, Vol.5(9), pp.e12979-e12979
09/24/2010
Handle:
https://hdl.handle.net/2376/117111
PMCID: PMC2945314
PMID: 20886044
Abstract
Primordial follicle assembly is a process that occurs when oocyte nests break down to form individual primordial follicles. The size of this initial pool of primordial follicles in part determines the reproductive lifespan of the female. Connective tissue growth factor (CTGF) was identified as a potential regulatory candidate for this process in a previous microarray analysis of follicle development. The current study examines the effects of CTGF and associated transforming growth factor beta 1 (TGFβ-1) on follicle assembly. Ovaries were removed from newborn rat pups and placed in an organ culture system. The ovaries treated with CTGF for two days were found to have an increased proportion of assembled follicles. CTGF was found to regulate the ovarian transcriptome during primordial follicle assembly and an integrative network of genes was identified. TGFβ-1 had no effect on primordial follicle assembly and in combination with CTGF decreased oocyte number in the ovary after two days of culture. Over ten days of treatment only the combined treatment of CTGF and TGFβ-1 was found to cause an increase in the proportion of assembled follicles. Interestingly, treatment with TGFβ-1 alone resulted in fewer total oocytes in the ovary and decreased the primordial follicle pool size after ten days of culture. Observations indicate that CTGF alone or in combination with TGFβ-1 stimulates primordial follicle assembly and TGFβ-1 can decrease the primordial follicle pool size. These observations suggest the possibility of manipulating primordial follicle pool size and influencing female reproductive lifespan.
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Details
- Title
- Induction of ovarian primordial follicle assembly by connective tissue growth factor CTGF
- Creators
- Ryan Schindler - Center for Reproductive Biology, School Biological Sciences, Washington State University, Pullman, Washington, United States of AmericaEric NilssonMichael K Skinner
- Publication Details
- PloS one, Vol.5(9), pp.e12979-e12979
- Academic Unit
- Biological Sciences, School of
- Publisher
- United States
- Grant note
- R01 HD043093-01 / NICHD NIH HHS R01 DK043093 / NIDDK NIH HHS
- Identifiers
- 99900548360701842
- Language
- English
- Resource Type
- Journal article