Journal article
Induction of protective immunity by using Anaplasma marginale initial body membranes
Infection and immunity, Vol.59(9), pp.3199-3204
09/1991
Handle:
https://hdl.handle.net/2376/114521
PMCID: PMC258153
PMID: 1715323
Abstract
Anaplasma marginale initial bodies of the Norton Zimbabwe strain were disrupted and separated into two membrane fractions banding at 1.15 and 1.22 g/cm3 by sucrose density centrifugation. The membrane fractions differed in their morphology and polypeptide composition. Membranes banding at 1.22 g/cm3 shared epitopes with surface-exposed polypeptides of the Florida strain of A. marginale, confirming the outer membrane location of these polypeptides. Immunization of cattle with either membrane fraction induced protection against homologous challenge, as demonstrated by significantly less anemia and lower peak rickettsemia values compared with those of adjuvant-immunized and nonimmunized calves. Protection correlated with antibody titer to membrane polypeptides. Although both membrane fractions induced protection, a 31-kDa polypeptide was the only common antigen to both fractions, as shown by reactivity of immune sera. Identification of membrane antigens capable of inducing protective immunity should facilitate development of vaccines against anaplasmosis suitable for use in Zimbabwe.
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Details
- Title
- Induction of protective immunity by using Anaplasma marginale initial body membranes
- Creators
- N Tebele - Veterinary Research Laboratory, Hazare, ZimbabweT C McGuire - Veterinary Research Laboratory, Hazare, ZimbabweG H Palmer - Veterinary Research Laboratory, Hazare, Zimbabwe
- Publication Details
- Infection and immunity, Vol.59(9), pp.3199-3204
- Identifiers
- 99900548260201842
- Language
- English
- Resource Type
- Journal article