Induction of type 2 cytokines by a staphylococcal enterotoxin superantigen

W A Ferens; W L Goff; W C Davis; L K Fox; C Deobald; M J Hamilton; G A Bohach

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Induction of type 2 cytokines by a staphylococcal enterotoxin superantigen

Journal article

Peer reviewed

Induction of type 2 cytokines by a staphylococcal enterotoxin superantigen

W A Ferens, W L Goff, W C Davis, L K Fox, C Deobald, M J Hamilton and G A Bohach

Journal of natural toxins, Vol.7(3), pp.193-213

10/1998

Handle:

https://hdl.handle.net/2376/114711

PMID: 9783259

Abstract

Superantigens - toxicity

Enterotoxins - toxicity

Staphylococcal Infections - immunology

Cells, Cultured

RNA, Messenger - analysis

Mastitis, Bovine - etiology

CD4-Positive T-Lymphocytes - immunology

Interleukin-4 - immunology

RNA, Messenger - biosynthesis

Interleukin-4 - biosynthesis

Phenotype

Animals

Cattle

Cell Division - immunology

Female

Interleukin-10 - biosynthesis

Staphylococcus aureus - immunology

Mastitis, Bovine - immunology

CD8-Positive T-Lymphocytes - immunology

Interleukin-10 - immunology

Staphylococcal Infections - etiology

Persistent intramammary infections of dairy cows with Staphylococcus aureus may involve immunosuppression mediated by bacterial toxins such as enterotoxins and other super-antigens (SAgs). Previously we found that stimulation of bovine PBMC with staphylococcal enterotoxin C (SEC) induced a unique phenotype of activated CD8+ T cells expressing a newly identified activation molecule, ACT3. In the present study we found that SEC induced the expression of interleukin (IL)-4 and IL-10 mRNAs, two cytokines associated with type 2 responses. Elevated levels of IL-4 and IL-10, observed between day 0 and day 4 of culture, were associated with temporary inhibition of proliferative responses of T cells, evidenced by a decrease in numbers of CD4+ T cells and a small increase in numbers of CD8+ T cells. Vigorous proliferation of T cells occurred between days 4 and 7 of culture and with a bias towards CD8+ T cells. Acquisition of the ACT3+ phenotype by CD8+ T cells was preceded by induction of IL-4 mRNA. Thus, in the bovine system, SAgs may hinder protective responses by inducing type 2 cytokines, which interfere with immune clearance of many microbial pathogens. The results of the study are consistent with the hypothesis that SAgs are involved in immunosuppression, and suggest possible immunomodulatory mechanisms.

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Title: Induction of type 2 cytokines by a staphylococcal enterotoxin superantigen
Creators: W A Ferens - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow 83844, USA
W L Goff
W C Davis
L K Fox
C Deobald
M J Hamilton
G A Bohach
Publication Details: Journal of natural toxins, Vol.7(3), pp.193-213
Academic Unit: Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
Publisher: United States
Identifiers: 99900547686401842
Language: English
Resource Type: Journal article

Induction of type 2 cytokines by a staphylococcal enterotoxin superantigen

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