Journal article
Influenza virus pathophysiology and brain invasion in mice with functional and dysfunctional Mx1 genes
Brain, behavior, and immunity, Vol.26(1), pp.83-89
01/2012
Handle:
https://hdl.handle.net/2376/110256
PMCID: PMC3221813
PMID: 21821116
Abstract
Mice with a dysfunctional myxovirus resistance-1 (dMx1) gene transport intranasally-instilled PR8 influenza virus to the olfactory bulb (OB) within 4 h post-infection. To determine if the presence of a functional Mx1 (fMx1) gene would influence this brain viral localization and/or disease, we infected mature C57BL/6 dMx1 and fMx1 mice under the same conditions and observed sickness behaviors, viral nucleoprotein (NP) RNA expression and innate immune mediator (IIM) mRNA expression in selected tissues at 15 and 96 h post-infection. Virus invaded the OB and lungs comparably in both sub-strains at 15 and 96 h as determined by nested PCR. In contrast, virus was present in blood and somatosensory cortex of dMx1, but not fMx1 mice at 96 h. At 15 h, sickness behaviors were comparable in both sub-strains. By 96 h dMx1, but not fMx1, were moribund. In both 15 and 96 h lungs, viral NP was significantly elevated in the dMx1 mice compared to the fMx1 mice, as determined by quantitative PCR. OB expression of most IIM mRNAs was similar at both time periods in both sub-strains. In contrast, lung IIM mRNAs were elevated in fMx1 at 15 h, but by 96 h were consistently reduced compared to dMx1 mice. In conclusion, functional Mx1 did not alter OB invasion by virus but attenuated illness compared to dMx1 mice. Inflammation was similar in OBs and lungs of both strains at 15 h but by 96 h it was suppressed in lungs, but not in OBs, of fMx1 mice.
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Details
- Title
- Influenza virus pathophysiology and brain invasion in mice with functional and dysfunctional Mx1 genes
- Creators
- Nicole R Hodgson - Sleep and Performance Research Center, WWAMI Medical Education Program, Washington State University, Spokane, WA 99210-1495, United StatesStewart G BohnetJeannine A MajdeJames M Krueger
- Publication Details
- Brain, behavior, and immunity, Vol.26(1), pp.83-89
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Netherlands
- Grant note
- NS031453 / NINDS NIH HHS R01 HD036520-14 / NICHD NIH HHS R01 NS025378 / NINDS NIH HHS R01 NS031453-18 / NINDS NIH HHS R01 NS031453 / NINDS NIH HHS HD036520 / NICHD NIH HHS R01 HD036520 / NICHD NIH HHS
- Identifiers
- 99900547501301842
- Language
- English
- Resource Type
- Journal article