Journal article
Inheritance of Virulence and Linkages of Virulence Genes in an Ethiopian Isolate of the Wheat Stripe Rust Pathogen ( Puccinia striiformis f. sp. tritici ) Determined Through Sexual Recombination on Berberis holstii
Plant disease, Vol.103(9), pp.PDIS02190269RE-2459
07/19/2019
Handle:
https://hdl.handle.net/2376/109029
PMID: 31322491
Abstract
Stripe rust, caused by
f. sp.
, is one of the most devastating wheat diseases in Ethiopia. To study virulence genetics of the pathogen, 117 progeny isolates were produced through sexual reproduction of an Ethiopian isolate of the stripe rust pathogen on
plants under controlled conditions. The parental and progeny isolates were characterized by phenotyping on wheat lines carrying single
genes for resistance and genotyped using 10 polymorphic simple sequence repeated (SSR) markers. The progeny isolates were classified into 37 virulence phenotypes and 75 multilocus genotypes. The parental isolate and progeny isolates were all avirulent to resistance genes
,
,
,
,
,
,
, and
but virulent to
and
, indicating that the parental isolate was homozygous avirulent or homozygous virulent at these loci. The progeny isolates segregated for virulence to 12
genes. Virulence phenotypes to
,
,
, and
were controlled by a single dominant gene; those to
,
,
,
,
,
, and
were each controlled by two dominant genes; and the virulence phenotype to
was controlled by two complementary dominant genes. A linkage map was constructed with seven SSR markers, and 16 virulence loci corresponding to 11
resistance genes were mapped with some loci linked to each other. These results are useful in understanding host-pathogen interactions and selecting resistance genes to develop wheat cultivars with highly effective resistance to stripe rust.
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Details
- Title
- Inheritance of Virulence and Linkages of Virulence Genes in an Ethiopian Isolate of the Wheat Stripe Rust Pathogen ( Puccinia striiformis f. sp. tritici ) Determined Through Sexual Recombination on Berberis holstii
- Creators
- Gebreslasie Zeray Siyoum - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaQingdong Zeng - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaJie Zhao - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaXianming Chen - 2Wheat Health, Genetics, and Quality Research Unit, U.S. Department of Agriculture Agricultural Research Service, Pullman, WA 99164-6430, U.S.AAyele Badebo - 3International Maize and Wheat Improvement Center (CIMMYT) Ethiopia, International Livestock Research Institute Gurd Shola Campus, Addis Ababa, EthiopiaYuan Tian - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaLili Huang - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaZhensheng Kang - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of ChinaGangming Zhan - 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
- Publication Details
- Plant disease, Vol.103(9), pp.PDIS02190269RE-2459
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900547187601842
- Language
- English
- Resource Type
- Journal article