Journal article
Inhibition of Protein Tyrosine Phosphatases by Low-Molecular-Weight S-Nitrosothiols and S-Nitrosylated Human Serum Albumin
Biochemical and biophysical research communications, Vol.268(2), pp.310-314
02/16/2000
Handle:
https://hdl.handle.net/2376/113931
PMID: 10679200
Abstract
The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order rate constants (kinact/KI) ranging from 37 to 113 M−1 min−1 against mammalian PTP1B and from 66 to 613 M−1 min−1 against Yersinia PTPase. Furthermore, the inactivation of Yersinia PTPase by S-nitrosylated protein:S-nitroso human serum albumin was investigated. Both single-S-nitrosylated and poly-S-nitrosylated human serum albumin show good inhibitory ability to Yersinia PTPase. The second-order rate constants are 472 and 1188 M−1 min−1, respectively. This result indicates a possibility that S-nitrosylated albumin in vivo may function as an inhibitor for a variety of cysteine-dependent enzymes.
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Details
- Title
- Inhibition of Protein Tyrosine Phosphatases by Low-Molecular-Weight S-Nitrosothiols and S-Nitrosylated Human Serum Albumin
- Creators
- Ming Xian - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Kun Wang - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Xinchao Chen - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Yongchun Hou - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Andrea McGill - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Xi Chen - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202Bo Zhou - Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461Zhong-Yin Zhang - Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461Jin-Pei Cheng - Department of Chemistry, Nankai University, Tianjin, 300071, ChinaPeng George Wang - Department of Chemistry, Wayne State University, Detroit, Michigan, 48202
- Publication Details
- Biochemical and biophysical research communications, Vol.268(2), pp.310-314
- Academic Unit
- Chemistry, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547938101842
- Language
- English
- Resource Type
- Journal article