Inhibitory actions of Anti-Müllerian Hormone (AMH) on ovarian primordial follicle assembly

Eric E Nilsson; Ryan Schindler; Marina I Savenkova; Michael K Skinner

doi:10.1371/journal.pone.0020087

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Inhibitory actions of Anti-Müllerian Hormone (AMH) on ovarian primordial follicle assembly

Journal article

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Inhibitory actions of Anti-Müllerian Hormone (AMH) on ovarian primordial follicle assembly

Eric E Nilsson, Ryan Schindler, Marina I Savenkova and Michael K Skinner

PloS one, Vol.6(5), pp.e20087-e20087

2011

DOI: https://doi.org/10.1371/journal.pone.0020087

Handle:

https://hdl.handle.net/2376/107907

PMCID: PMC3103528

PMID: 21637711

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Abstract

Gene Regulatory Networks - genetics

Oocytes - metabolism

Cell Count

Anti-Mullerian Hormone - pharmacology

Gene Expression Profiling

Signal Transduction - genetics

Rats, Sprague-Dawley

Gene Expression Regulation - drug effects

Oocytes - cytology

Ovarian Follicle - drug effects

Signal Transduction - drug effects

Oocytes - drug effects

Female

Anti-Mullerian Hormone - metabolism

Ovarian Follicle - cytology

Ovarian Follicle - metabolism

Progesterone - pharmacology

Transforming Growth Factor beta - metabolism

Immunohistochemistry

The current study was designed to investigate the actions of Anti-Müllerian Hormone (AMH) on primordial follicle assembly. Ovarian primordial follicles develop from the breakdown of oocyte nests during fetal development for the human and immediately after birth in rodents. AMH was found to inhibit primordial follicle assembly and decrease the initial primordial follicle pool size in a rat ovarian organ culture. The AMH expression was found to be primarily in the stromal tissue of the ovaries at this period of development, suggesting a stromal-epithelial cell interaction for primordial follicle assembly. AMH was found to promote alterations in the ovarian transcriptome during primordial follicle assembly with over 200 genes with altered expression. A gene network was identified suggesting a potential central role for the Fgf2/Nudt6 antisense transcript in the follicle assembly process. A number of signal transduction pathways are regulated by AMH actions on the ovarian transcriptome, in particular the transforming growth factor-beta (TGFß) signaling process. AMH is the first hormone/protein shown to have an inhibitory action on primordial follicle assembly. Due to the critical role of the primordial follicle pool size for female reproduction, elucidation of factors, such as AMH, that regulate the assembly process will provide insights into potential therapeutics to manipulate the pool size and female reproduction.

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Title: Inhibitory actions of Anti-Müllerian Hormone (AMH) on ovarian primordial follicle assembly
Creators: Eric E Nilsson - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, Washington, United States of America
Ryan Schindler
Marina I Savenkova
Michael K Skinner
Publication Details: PloS one, Vol.6(5), pp.e20087-e20087
Academic Unit: Biological Sciences, School of
Publisher: United States
Identifiers: 99900546817701842
Language: English
Resource Type: Journal article